申请试用 Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb (S-1389-148)（Discontinued, Recommend Catalog No.S0B6839 ）
Transcription factor p65,Nuclear factor NF-kappa-B p65 subunit,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3,RELA,NFKB3
分享产品介绍 FAQs 引用文献(1) 评论(0)
宿主来源
Rabbit抗原名称
Phospho-NF-κB p65 (Ser536)分子别名
Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; RELA; NFKB3免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q04206克隆号
S-1389-148抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms阳性样本
HeLa treated with 100 ng/ml Calyculin A for 30 minutes and 20 ng/ml TNF-α for 5 minutes, NIH/3T3 treated with 20 ng/ml TNF-α for 10 minutes and 100 nM Calyculin A for 10 minutes纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
应用
WB稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Hu, Ms
Phospho-NF-κB p65 (Ser536) refers to the phosphorylation of the p65 subunit of the NF-κB protein complex at the serine 536 residue. This post-translational modification is crucial for regulating the transcriptional activity, nuclear localization, and protein stability of NF-κB, which is a master regulator of inflammation and cell survival. Phosphorylation at Ser536 has been shown to negatively regulate NF-κB p65 activity. In a study published in Sci Signal, it was demonstrated that mice expressing a mutant p65 with an alanine-to-serine substitution at position 534 (the murine homolog of human Ser536) exhibited increased expression of NF-κB-dependent genes upon inflammatory stimulation, leading to enhanced inflammation and increased mortality. The phosphorylation status of NF-κB p65 at Ser536 is also associated with the prognosis of certain cancers. For instance, in colorectal cancer, increased expression of phospho-Ser536-p65 in the cytoplasm of primary tumors correlates with worse patient survival, independent of other clinical factors, making it an independent prognostic factor. Moreover, the phosphorylation of NF-κB p65 at Ser536 has been implicated in the pathogenesis of liver cancer.
免疫印迹
WB result of Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb
Primary antibody: Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes and 20 ng/ml TNF-α for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 65 kDaWB result of Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb
Primary antibody: Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 20 ng/ml TNF-α for 10 minutes and 100 nM Calyculin A for 10 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 65 kDa
斑点杂交
Dot blot result of Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb
Lane 1: NF-κB p65 (Ser536) phospho peptide
Lane 2: NF-κB p65 unmodified peptide
Primary antibody: Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
引用文献(1)
- A novel mechanism of Reduning injection in sepsis treatment: targeting inflammatory kinases TBK1 and IKKβ
L Cao, ZY Wu, Y Gao, X Geng, W Yang, Q Yin, Q Liu… - Phytomedicine, 2026 - Elsevier
Phytomedicine. 2026 Jun 06 .
影响因子： 8.3
货号：S0B1035产品名称：Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb (S-1389-148)
评论(0)