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Rabbit抗原名称
Musashi 1/Msi1分子别名
RNA-binding protein Musashi homolog 1免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
O43347克隆号
S-1477-58抗体类型
Recombinant mAb抗体同种型
IgG应用
ICFCM, ICC, WB反应种属 ?
Hu, Ms阳性样本
SH-SY5Y, Neuro-2a预测反应种属
(反应种属缩写表)Rt纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:500 | Hu, Ms |
| ICC | 1:500 | Hu, Ms |
| ICFCM | 1:50 | Hu |
Musashi-1 is an evolutionarily conserved RNA-binding protein that is selectively expressed in neural stem/progenitor cells within the central nervous system (CNS). It plays a crucial role in the self-renewal and differentiation of these cells by regulating various target mRNAs, including those encoding for Numb and p21(CIP-1). As a transcriptional repressor, Musashi-1 can directly modulate the expression of its target proteins, which are involved in critical cellular processes such as cell proliferation, apoptosis, and tumorigenesis. It has been found to be overexpressed in various solid tumors, including neuroglioma, esophageal, gastric, colorectal, and breast cancers. This overexpression suggests that Musashi-1 may contribute to tumor development and maintenance, possibly by sustaining the stem-like properties of cancer cells and promoting their proliferation. Musashi-1 functions by binding to specific mRNAs and regulating their translation. It can act as a translational suppressor of Numb mRNA and synergistically regulate the Notch signaling pathway, which is important for asymmetric cell division in stem cells. This protein's role in maintaining stem cell functions and its involvement in tumor-related signaling pathways make it a significant protein in the context of cancer research and potential therapeutic targets.
免疫印迹
WB result of Musashi 1/Msi1 Recombinant Rabbit mAb
Primary antibody: Musashi 1/Msi1 Recombinant Rabbit mAb at 1/500 dilution
Lane 1: SH-SY5Y whole cell lysate 40 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 39 kDaWB result of Musashi 1/Msi1 Recombinant Rabbit mAb
Primary antibody: Musashi 1/Msi1 Recombinant Rabbit mAb at 1/500 dilution
Lane 1: NIH/3T3 whole cell lysate 40 µg
Lane 2: Neuro-2a whole cell lysate 40 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 39 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) labelling Musashi 1 / Msi1 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in SH-SY5Y cells. Anti-Musashi 1 / Msi1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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