Invivo anti-mouse CD8β (Lyt 3.2) Recombinant mAb
CD8B,CD8B1,T-lymphocyte differentiation antigen T8/Leu-2,T-cell surface glycoprotein CD8 beta chain,Lymphocyte antigen 3,T-cell membrane glycoprotein Ly-3,T-cell surface glycoprotein Lyt-3,CD8b
货号： S0B1032
- 价格： ￥1,788
- 规格：
- 数量：
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宿主来源
Rat抗原名称
CD8β (Lyt 3.2)分子别名
T-cell surface glycoprotein CD8 beta chain; Lymphocyte antigen 3; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein Lyt-3, CD8b细胞定位
MembraneAccession
P10300克隆号
S-R484抗体类型
Rat mAb抗体同种型
Rat IgG1,k应用
in vivo CD8+ T cell depletion, FCM反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
5 mg/ml内毒素含量
<2EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 1:5000 | Ms |
The CD8 antigen is a cell surface glycoprotein primarily found on most cytotoxic T lymphocytes (CTLs). It acts as a co-receptor, along with the T-cell receptor (TCR), to recognize antigens presented by antigen-presenting cells (APCs) in the context of class I MHC molecules. The functional co-receptor can be either a homodimer composed of two alpha chains or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology with immunoglobulin variable light chains. In research, CD8B plays a central role in the development and function of T cells. For example, it is involved in the positive and negative selection processes of T cells in the thymus, as well as the regulation of mature single-positive CD8+ T cells being released into the circulation. Moreover, the expression of the CD8B gene is finely regulated, including the regulation of CD4 and CD8A gene expression during thymic differentiation.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling mouse CD8β (Lyt 3.2) antibody at 1/5000 (0.1 μg) dilution (Right) compared with a Rat monoclonal IgG isotype control (Left). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD8α - Alexa Fluor® 647 separately. Gated on total viable cells.
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling mouse CD8β (Lyt 3.2) antibody at 1/5000 (0.1 μg) dilution (Right) compared with a Rat monoclonal IgG isotype control (Left). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Brilliant Violet 421™ separately. Gated on total viable cells.
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