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Rat Anti-Mouse CD45R/B220 Antibody (RA3-6B2)

Receptor-type tyrosine-protein phosphatase C,Leukocyte common antigen (L-CA),Lymphocyte antigen 5 (Ly-5),T200,Ptprc,Ly-5

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1031

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rat
抗原名称

CD45R/B220
分子别名

Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); Lymphocyte antigen 5 (Ly-5); T200; Ptprc; Ly-5
细胞定位

Cell membrane
Accession

P06800
克隆号

RA3-6B2
抗体类型

Rat mAb
抗体同种型

IgG2a,k
反应种属 ?

Ms
阳性样本

C57BL/6 mouse splenocytes, mouse spleen
纯化方式

Protein G
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

FCM

IF ?
稀释度

应用稀释度推荐种属

IHC-P 1:500 Ms
IF 1:500 Ms
FCM 1:2000 Ms

应用	稀释度	推荐种属
IHC-P	1:500	Ms
IF	1:500	Ms
FCM	1:2000	Ms

背景介绍

CD45R, also known as B220, is a protein tyrosine phosphatase that is predominantly found on B cells, at varying levels from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. CD45R plays a crucial role in B cell receptor and T cell receptor signaling and is essential for lymphocyte development and function. It helps regulate the threshold of sensitivity to external stimuli, affecting cellular responses. In mice, CD45R/B220 is a common marker for B cells, whereas in humans, B cells do not express B220 in the same way; it appears to be differentiation-specific and is therefore expressed on only some B cell subsets. CD45R is also used in the classification of leukemias and lymphomas and in research on functional subsets of B and T cells.

流式分析
- Flow cytometric analysis of C57BL/6 mouse splenocytes labeling Mouse CD45R/B220 at 1/2000 (0.1 μg) dilution / (Right panel) compared with a Rat IgG, Isotype Control / (Left panel). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Brilliant Violet 421™ separately. Gated on total viable cells.
免疫组化
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Mouse CD45R/B220 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-Mouse CD45R/B220 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse spleen. Anti-Mouse CD45R/B220 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rat IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱