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Rabbit抗原名称
Phospho-PDK1/PDPK1-S241分子别名
3-phosphoinositide-dependent protein kinase 1免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
O15530克隆号
S-1426-93抗体类型
Recombinant mAb抗体同种型
IgG应用
WB反应种属 ?
Ms阳性样本
NIH/3T3预测反应种属
(反应种属缩写表)Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Ms |
Phospho-PDK1 (Ser241) refers to the protein PDK1 that is phosphorylated at the Ser241 site. PDK1, full name 3-phosphoinositide-dependent protein kinase-1, is a serine/threonine protein kinase that plays a central role in regulating cell growth, survival, and proliferation. Overexpression of PDK1 in cells has been observed in breast and ovarian cancers, and its activity increases in prion-infected neurons. Phosphorylation of a conserved serine (S241) in the activation loop leads to the activation of PDK1.
免疫印迹
WB result of Phospho-PDK1/PDPK1-S241 Recombinant Rabbit mAb
Primary antibody: Phospho-PDK1/PDPK1-S241 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 starve overnight, then treated with 50 ng/ml PDGF for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 50~60 kDa
斑点杂交
Dot blot result of Phospho-PDK1/PDPK1-S241 Recombinant Rabbit mAb
Lane 1: PDK1/PDPK1-S241 phospho peptide
Lane 2: PDK1/PDPK1 unmodified peptide
Primary antibody: Phospho-PDK1/PDPK1-S241 Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
This blot was developed with high sensitivity substrate
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