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Rat克隆号
S-R486抗体类型
Rat mAb抗体同种型
IgG2b,k纯化方式
Protein G浓度
0.2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
FCM
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labeling Rat mAb IgG2b Isotype Control at 1/20 dilution (1 μg) / (Left panel) compared with CD8α antibody at 1/200 (1 μg) dilution (S0B0498) / (Right panel). Goat Anti-Rat IgG Alexa Fluor 488 was used as the secondary antibody. Then cells were stained with CD4 - Brilliant Violet 421™ separately. Gated on total viable cells.
免疫组化
IHC shows negative staining in paraffin-embedded mouse spleen. Rat mAb IgG2b Isotype Control was used at 1/80 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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