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宿主来源
Rabbit抗原名称
5HT1A分子别名
5-hydroxytryptamine receptor 1A; 5-HT-1A; 5-HT1A; G-21; Serotonin receptor 1A; HTR1A; ADRB2RL1; ADRBRL1免疫原
Synthetic Peptide细胞定位
Cell membrane, Cell projectionAccession
P08908抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
mouse brain, rat brain预测反应种属
(反应种属缩写表)Dg, Cz, Hr, Or, Gor纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:50 Hu, Ms, Rt
5-HT1A, also known as the serotonin 1A receptor, is a G protein-coupled receptor that plays a significant role in regulating a variety of physiological processes and has been implicated in several pathologies, particularly in the realm of mental health1. It is highly expressed in the brain, particularly in regions such as the hippocampus, septum, and prefrontal cortex, and is known to modulate serotonin activity, which in turn influences mood, anxiety, and other behaviors. The 5-HT1A receptor functions as both a presynaptic autoreceptor, inhibiting the release of serotonin, and as a postsynaptic heteroreceptor, modulating the effects of serotonin in the brain. It is also found on the cell bodies and dendrites of neurons, where it can influence neuronal excitability and synaptic transmission.
免疫印迹
WB result of 5HT1A Receptor Rabbit pAb
Primary antibody: 5HT1A Receptor Rabbit p Ab at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 46 kDaWB result of 5HT1A Receptor Rabbit pAb
Primary antibody: 5HT1A Receptor Rabbit p Ab at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 46 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-5HT1A antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hippocampus. Anti-5HT1A antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-5HT1A antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-5HT1A antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of 5HT1A in tumor tissue.
Expression of 5HT1A in human tissue.
Expression of 5HT1A in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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