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宿主来源
Rabbit抗原名称
SOD2/MnSOD免疫原
Synthetic PeptideAccession
P04179抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HEK-293, HepG2, SH-SY5Y, NIH/3T3, RAW264.7, mouse heart, mouse brain, rat heart, rat brain预测反应种属
(反应种属缩写表)Bv, Mq, Or, GP纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:10000 Hu, Ms, Rt IHC-P 1:500 Hu, Ms, Rt
SOD2, or manganese superoxide dismutase, is a critical enzyme found in the mitochondria of almost all cells in the body. Its primary function is to dismutate superoxide radicals into molecular oxygen and hydrogen peroxide, thus protecting the body from the harmful effects of free radicals. SOD2 is essential for maintaining cellular homeostasis and is implicated in a variety of biological processes, including aging, cancer, and neurodegenerative diseases. In the context of cancer, SOD2 has been linked to tumor progression. It is often upregulated in various cancer types, including lung, breast, colon, and brain cancers, and is associated with poor prognosis, distant metastasis, and lower overall survival rates. SOD2 also plays a significant role in mitochondrial function and has been implicated in mitochondrial dysfunction and neurodegeneration. It is suggested that SOD2, by converting superoxide radicals to hydrogen peroxide, can modulate cellular signaling pathways involved in cell survival, death, and differentiation. Moreover, SOD2 has been studied for its potential role in radiotherapy resistance.
免疫印迹
WB result of SOD2/MnSOD Rabbit pAb
Primary antibody: SOD2/MnSOD Rabbit p Ab at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 22 kDaWB result of SOD2/MnSOD Rabbit pAb
Primary antibody: SOD2/MnSOD Rabbit p Ab at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse heart lysate 5 µg
Lane 4: mouse brain lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 22 kDaWB result of SOD2/MnSOD Rabbit pAb
Primary antibody: SOD2/MnSOD Rabbit p Ab at 1/10000 dilution
Lane 1: rat heart lysate 5 µg
Lane 2: rat brain lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 22 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-SOD2/MnSOD antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-SOD2/MnSOD antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-SOD2/MnSOD antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-SOD2/MnSOD antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-SOD2/MnSOD antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-SOD2/MnSOD antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of SOD2/MnSOD in tumor tissue.
Expression of SOD2/MnSOD in human tissue.
Expression of SOD2/MnSOD in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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