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宿主来源
Rabbit抗原名称
Keratin 6分子别名
Keratin, type II cytoskeletal 6C, Cytokeratin-6C, CK-6C, KRT6C Cytokeratin-6E, CK-6E, KRT6E免疫原
Synthetic PeptideAccession
P48668克隆号
SDT-015-69抗体类型
Rabbit mAb抗体同种型
IgG2b反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
1 mg/ml分子量
60kDa
标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt, 4°C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000-1:25000 Hu, Ms IHC-P 1:1000 Hu, Ms, Rt
Keratin 6C is a type II cytokeratin, one of a number of isoforms of keratin 6 encoded by separate genes located within the type II keratin gene cluster on human chromosome 12q. Keratins are the intermediate filament proteins that form a dense meshwork of filaments throughout the cytoplasm of epithelial cells. Keratins form heteropolymers consisting of a type I and a type II keratin. Keratins are generally expressed in particular pairs of type I and type II keratin proteins in a tissue-specific and cellular differentiation-specific manner.
免疫印迹
WB result of anti-Keratin 6 antibody
Primary antibody : Anti-Keratin 6 antibody at 1/25000 dilution
Lane 1 : Hela whole cell lysate 20 µg
Lane 2 : A431 whole cell lysate 20 µg
Negative control: Hela whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
econds
WB result of anti-Keratin 6 antibody
Primary antibody : Anti-Keratin 6 antibody at 1:5000 dilution
Lane 1 : mouse skin lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
econds
免疫组化
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Keratin 6 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Keratin 6 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-Keratin 6 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows membrane staining in paraffin-embedded human tonsil. Anti-Keratin 6 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung adenocarcinoma. Anti-Keratin 6 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows membrane staining in paraffin-embedded mouse skin. Anti-Keratin 6 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows membrane staining in paraffin-embedded rat skin. Anti-Keratin 6 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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