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Rabbit抗原名称
YWHAH/14-3-3 eta分子别名
Protein AS1免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
Q04917克隆号
S-1327-22抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Jurkat, HeLa, A431, NIH/3T3, PC-12预测反应种属
(反应种属缩写表)Pr, Bv, Cz, Pg, Hm, Av, Hm纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:10000 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt ICC 1:500 Hu, Ms
The 14-3-3 η protein is a member of the 14-3-3 protein family, which plays a significant role in various cellular processes, including signal transduction, cell cycle regulation, and apoptosis. This protein is found in high concentrations in certain tissues and has been identified as a potential biomarker for rheumatoid arthritis (RA). In the context of RA, 14-3-3 η protein has been found to be elevated in the blood and synovial fluid of patients with RA compared to healthy individuals. It is considered a promising diagnostic biomarker that can complement existing serological measurements like rheumatoid factor (RF) and anticyclic citrullinated peptide antibody (ACPA) to enhance the detection of RA. Furthermore, 14-3-3 η protein levels have been correlated with disease activity and joint damage in RA. It has been suggested that the protein may be involved in the pathogenesis of RA by stimulating the expression of factors that contribute to joint damage, such as matrix metalloproteinases (MMPs) and receptor activator of nuclear factor kappa-B ligand (RANKL).
免疫印迹
WB result of YWHAH/14-3-3 eta Recombinant Rabbit mAb
Primary antibody: YWHAH/14-3-3 eta Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDaWB result of YWHAH/14-3-3 eta Recombinant Rabbit mAb
Primary antibody: YWHAH/14-3-3 eta Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDaWB result of YWHAH/14-3-3 eta Recombinant Rabbit mAb
Primary antibody: YWHAH/14-3-3 eta Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-YWHAH/14-3-3 eta antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-YWHAH/14-3-3 eta antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-YWHAH/14-3-3 eta antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-YWHAH/14-3-3 eta antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-YWHAH/14-3-3 eta antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-YWHAH/14-3-3 eta antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- YWHAH/14-3-3 eta antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- YWHAH/14-3-3 eta antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of YWHAH/14-3-3 eta in tumor tissue.
Expression of YWHAH/14-3-3 eta in human tissue.
Expression of YWHAH/14-3-3 eta in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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