申请试用 
分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
ATG12分子别名
Ubiquitin-like protein ATG12; Autophagy-related protein 12 (APG12-like); APG12; APG12L免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
O94817克隆号
S-1143-209抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HeLa, HCT 116, PANC-1预测反应种属
(反应种属缩写表)Rt, Bv, Or纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM ,IHC-P ? ,ICC ? ,WB稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:100 Hu, Ms ICC 1:500 Hu ICFCM 1:50 Hu
ATG12 protein is a crucial factor in the process of autophagy, serving as a human homolog of yeast autophagy-related genes. It plays an essential role in the formation of autophagosomes, the double-membrane structures that engulf and degrade cellular components. ATG12 forms a complex with ATG5 by being conjugated to it through the action of E1-like enzyme ATG7 and E2-like enzyme ATG10, and then this complex non-covalently binds with ATG16 to form the ATG12-ATG5-ATG16 complex, which is vital for the expansion of the autophagosome membrane. In terms of pathology, the expression of the ATG12 protein is associated with various diseases. For instance, reduced expression of ATG12 in cervical cancer tissue may be involved in the development of cervical cancer. Furthermore, the expression level of ATG12 correlates with the risk of recurrence after cervical conization surgery, with lower expression potentially increasing the risk of persistent or recurrent lesions.
免疫印迹
WB result of ATG12 Recombinant Rabbit mAb
Primary antibody: ATG12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HCT 116whole cell lysate 20 µg
Lane 3: PANC-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 20, 56 kDa
流式分析
Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) labelling ATG12 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-ATG12 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-ATG12 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-ATG12 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-ATG12 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC analysis of HeLa cells treated with chloroquine (50uM, 24 h) (top panel) and HeLa cells untreated with chloroquine (50uM, 24 h) (below panel). Anti- ATG12 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of ATG12 in tumor tissue.
Expression of ATG12 in human tissue.
Expression of ATG12 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)