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宿主来源
Mouse抗原名称
CD117分子别名
Piebald trait protein (PBT); Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; KIT; SCFR细胞定位
Cytoplasm, MembraneAccession
P10721克隆号
S-R470-1抗体类型
Mouse mAb抗体同种型
IgG1,k应用
FCM反应种属 ?
Hu阳性样本
human PBMC纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4储存条件
12 months from date of receipt / reconstitution, 2-8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 1:2000 | Hu |
Proto-oncogene c-KIT is the gene encoding the receptor tyrosine kinase protein known as tyrosine-protein kinase KIT, CD117 (cluster of differentiation 117) or mast/stem cell growth factor receptor (SCFR). Altered forms of this receptor may be associated with some types of cancer. KIT is a receptor tyrosine kinase type III, which binds to stem cell factor, also known as "steel factor" or "c-kit ligand" and expressed on the surface of hematopoietic stem cells as well as other cell types. When this receptor binds to stem cell factor (SCF) it forms a dimer that activates its intrinsic tyrosine kinase activity, that in turn phosphorylates and activates signal transduction molecules that propagate the signal in the cell. After activation, the receptor is ubiquitinated to mark it for transport to a lysosome and eventual destruction. Signaling through KIT plays a role in cell survival, proliferation, and differentiation. For instance, KIT signaling is required for melanocyte survival, and it is also involved in haematopoiesis and gametogenesis.
流式分析
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling Human CD117 antibody at 1/2000 (0.1 μg) dilution (Right) compared with a Mouse monoclonal IgG isotype control (Left). Goat Anti - Mouse IgG Phycoerythrin was used as the secondary antibody. Then cells were stained with CD34 - APC separately. Events were gated on total viable cells.
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