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宿主来源
Rabbit抗原名称
Flotillin 1分子别名
FLOT1免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
O75955抗体同种型
IgG反应种属 ?
Hu阳性样本
SK-OV-3, 293T, A431, C2C12, C6, PC-12预测反应种属
(反应种属缩写表)Mq, Or, Pg, Bv纯化方式
Protein A浓度
Lot specific* (generally 0.2 to 2 mg/ml)*标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu
Flotillin-1 is a lipid raft marker protein that is involved in a variety of cellular physiological activities and is associated with the development of many types of cancer. In terms of cellular functions, Flotillin-1 helps maintain cell membrane structure, participates in signal transduction, is involved in endocytosis, and plays a role in cell adhesion. It interacts with various signaling molecules to form signaling complexes that regulate intracellular signaling pathways. For example, it associates with Src kinase and is involved in processes such as cell growth, differentiation, and apoptosis. Flotillin-1 has also been implicated in the pathology of several diseases. It is highly expressed in lung adenocarcinoma, breast cancer, and hepatocellular carcinoma, and its expression is associated with poor prognosis and low overall survival rates. Flotillin-1 is involved in the activation of signaling pathways, such as the IGF-1 receptor, and it plays a role in the intracellular localization and signaling function of this receptor. Moreover, Flotillin-1 has been shown to be essential for the efficient sorting of T cell receptors into Rab11a vesicles for fusion with the plasma membrane, indicating its role in immune cell function. In terms of neurological diseases, Flotillin-1 has been linked to neurodegenerative conditions such as Parkinson's disease, where its expression levels are significantly elevated in the substantia nigra/ventral tegmental area.
免疫印迹
WB result of Flotillin 1 Rabbit pAb
Primary antibody: Flotillin 1 Rabbit pAb at 1/1000 dilution
Lane 1: SK-OV-3 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDaWB result of Flotillin 1 Rabbit pAb
Primary antibody: Flotillin 1 Rabbit pAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDaWB result of Flotillin 1 Rabbit pAb
Primary antibody: Flotillin 1 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti- Flotillin 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti- Flotillin 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti- Flotillin 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti- Flotillin 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti- Flotillin 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Flotillin 1 in tumor tissue
Expression of Flotillin 1 in human tissue
Expression of Flotillin 1 in mouse & rat tissue
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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