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Rabbit抗原名称
Beta IV Tubulin分子别名
Tubulin beta-4A chain, Tubulin 5 beta, Tubulin beta-4 chain, TUBB4A, TUBB4, TUBB5免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
P04350抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, Jurkat, Neuro-2a, mouse brain, C6, rat brain纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000-1:50000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt ICC 1:4000 Hu, Ms
Beta IV Tubulin, also known as tubulin beta 4A class IVa or TUBB4, is a specific isotype of beta-tubulin that plays a crucial role in the central nervous system. It is selectively expressed by oligodendrocytes, which are the cells responsible for myelination in the brain and spinal cord. This tubulin isotype is important for maintaining the structure and function of oligodendrocytes and their ability to form myelin. Myelin is a fatty substance that insulates nerve fibers and is essential for the proper conduction of nerve impulses. Beta IV Tubulin may also be involved in cell signaling pathways and gene expression patterns that change during oligodendrocyte differentiation and myelination.
免疫印迹
WB result of Beta IV Tubulin Rabbit pAb
Primary antibody: Beta IV Tubulin Rabbit pAb at 1/50000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDaWB result of Beta IV Tubulin Rabbit pAb
Primary antibody: Beta IV Tubulin Rabbit pAb at 1/50000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDaWB result of Beta IV Tubulin Rabbit pAb
Primary antibody: Beta IV Tubulin Rabbit pAb at 1/50000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti- Beta IV Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti- Beta IV Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Beta IV Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti- Beta IV Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- Beta IV Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-Beta IV Tubulin antibody was used at 1/4000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in Neuro-2a cells. Anti-Beta IV Tubulin antibody was used at 1/4000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
组织表达图谱
Expression of Beta IV Tubulin in tumor tissue
Expression of Beta IV Tubulin in human tissue
Expression of Beta IV Tubulin in mouse & rat tissue
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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