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Beta IV Tubulin Rabbit polyclonal antibody

Tubulin beta-4A chain,Tubulin 5 beta,Tubulin beta-4 chain,TUBB4A,TUBB4,TUBB5

货号： S0B0995

Datasheet COA
  • 价格： ￥400
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Beta IV Tubulin

  • 分子别名

    Tubulin beta-4A chain, Tubulin 5 beta, Tubulin beta-4 chain, TUBB4A, TUBB4, TUBB5

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Cytoskeleton

  • Accession

    P04350

  • 抗体同种型

    IgG

  • 应用

    IHC-P, ICC, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, Jurkat, Neuro-2a, mouse brain, C6, rat brain

  • 纯化方式

    Immunogen Affinity

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:50000 Hu, Ms, Rt
IHC-P 1:1000 Hu, Ms, Rt
ICC 1:4000 Hu, Ms
背景介绍

  • Beta IV Tubulin, also known as tubulin beta 4A class IVa or TUBB4, is a specific isotype of beta-tubulin that plays a crucial role in the central nervous system. It is selectively expressed by oligodendrocytes, which are the cells responsible for myelination in the brain and spinal cord. This tubulin isotype is important for maintaining the structure and function of oligodendrocytes and their ability to form myelin. Myelin is a fatty substance that insulates nerve fibers and is essential for the proper conduction of nerve impulses. Beta IV Tubulin may also be involved in cell signaling pathways and gene expression patterns that change during oligodendrocyte differentiation and myelination.

  • 免疫印迹

    • WB result of Beta IV Tubulin Rabbit pAb
      Primary antibody: Beta IV Tubulin Rabbit pAb at 1/50000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 50 kDa
      Observed MW: 52 kDa

    • WB result of Beta IV Tubulin Rabbit pAb
      Primary antibody: Beta IV Tubulin Rabbit pAb at 1/50000 dilution
      Lane 1: Neuro-2a whole cell lysate 20 µg
      Lane 2: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 50 kDa
      Observed MW: 52 kDa

    • WB result of Beta IV Tubulin Rabbit pAb
      Primary antibody: Beta IV Tubulin Rabbit pAb at 1/50000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: rat brain lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 50 kDa
      Observed MW: 52 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti- Beta IV Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti- Beta IV Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Beta IV Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse kidney. Anti- Beta IV Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- Beta IV Tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-Beta IV Tubulin antibody was used at 1/4000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    • ICC shows positive staining in Neuro-2a cells. Anti-Beta IV Tubulin antibody was used at 1/4000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

  • 组织表达图谱

    • Expression of Beta IV Tubulin in tumor tissue

    • Expression of Beta IV Tubulin in human tissue

    • Expression of Beta IV Tubulin in mouse & rat tissue

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