c-Fos Recombinant Rabbit mAb (S-1391-63)
Protein c-Fos,Cellular oncogene fos,Fos proto-oncogene AP-1 transcription factor subunit,G0/G1 switch regulatory protein 7,Proto-oncogene c-Fos,Transcription factor AP-1 subunit c-Fos,FOS,G0S7
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宿主来源
Rabbit抗原名称
c-Fos分子别名
Protein c-Fos, Cellular oncogene fos; Fos proto-oncogene AP-1 transcription factor subunit; G0/G1 switch regulatory protein 7; Proto-oncogene c-Fos; Transcription factor AP-1 subunit c-Fos; FOS; G0S7免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P01100克隆号
S-1391-63抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa预测反应种属
(反应种属缩写表)Hm, Ct, Sh纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IP 1:50 Hu IHC-P 1:250 Hu, Ms, Rt ICC 1:500 Hu
c-Fos is a nuclear phosphoprotein that plays a pivotal role in cell signaling, proliferation, and differentiation. It forms a heterodimer with c-Jun, which then forms the activator protein-1 (AP-1) complex. This complex binds to specific DNA sequences in the promoter and enhancer regions of target genes, transforming extracellular signals into changes in gene expression. c-Fos is crucial in regulating cell development and maintaining skeletal formation. The expression of c-Fos is associated with various biological processes, including cell proliferation, differentiation, and programmed cell death. Additionally, c-Fos is involved in learning and memory processes, with changes in its expression observed in brain regions associated with recognition, working, and fear-related memory. c-Fos is also used as a marker for neural activity, with its expression linked to changes in neuronal function and plasticity. The expression of c-Fos is also related to the occurrence and development of cancer. The c-Fos protein can serve as a marker for tumorigenesis, and its dysregulation is associated with cancer progression. The absence of c-Fos leads to significant neurological abnormalities in mice embryos during development, indicating that c-Fos plays an important role in neurogenesis and brain development.
免疫印迹
WB result of c-Fos Recombinant Rabbit mAb
Primary antibody: c-Fos Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa starve overnight, then treated with 200 nM PMA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 38~60 kDa
免疫沉淀
c-Fos Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating c-Fos in 0.4 mg HeLa starve overnight, 200nM PMA for 4 hours whole cell lysate whole cell lysate.
Western blot was performed on the immunoprecipitate using c-Fos Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa starve overnight, 200nM PMA for 4 hours whole cell lysate whole cell lysate 40 µg (Input)
Lane 2: c-Fos Recombinant Rabbit mAb IP in HeLa starve overnight, 200nM PMA for 4 hours whole cell lysate whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa starve overnight, 200nM PMA for 4 hours whole cell lysate whole cell lysate
Predicted MW: 41 kDa
Observed MW: 38~60 kDa
Exposure time: 20 s
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti- c-Fos antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti- c-Fos antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti- c-Fos antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti- c-Fos antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti- c-Fos antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC analysis of HeLa cells starve overnight then treated with 200nM PMA for 4 hours whole cell lysate and untreated HeLa cells (below panel). Anti- c-Fos antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of c-Fos in tumor tissue
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
引用文献(1)
- Veillonella intestinal colonization promotes C. difficile infection in Crohn's disease
Z Yang, J Wang, Y Chen, T Chen, Z Shen, Y Wang… - Cell Host & Microbe, 2025 - cell.com
Cell Host Microbe. 2025 Sep 10 .
影响因子： 18.7
货号：S0B0987产品名称：c-Fos Recombinant Rabbit mAb (S-1391-63)
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