HuD/ELAVL4 Recombinant Rabbit mAb (S-1351-159)

ELAV-like protein 4,Hu-antigen D,Paraneoplastic encephalomyelitis antigen HuD,PNEM

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0982

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

HuD/ELAVL4
分子别名

ELAV-like protein 4; Hu-antigen D; Paraneoplastic encephalomyelitis antigen HuD; PNEM
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P26378
克隆号

S-1351-159
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

SH-SY5Y
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB

IP

IF ?
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
IP 1:50 Hu
IHC-P 1:1000 Hu
ICC 1:500 Hu
IF 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu
IP	1:50	Hu
IHC-P	1:1000	Hu
ICC	1:500	Hu
IF	1:500	Hu

背景介绍

HuD, also known as ELAVL4, is an RNA-binding protein that is predominantly expressed in the brain and plays a crucial role in the development, maintenance, and functionality of the nervous system. It is involved in the regulation of gene expression at the post-transcriptional level and is known to interact with various types of RNAs, including mRNA, long non-coding RNA (lncRNA), and circular RNA (circRNA). HuD is implicated in several neurological disorders, including Parkinson’s disease, Alzheimer’s disease, and amyotrophic lateral sclerosis (ALS). Its altered expression or activity is associated with disease pathology, suggesting that HuD's regulatory functions on target transcripts can have significant implications for disease progression. In terms of cellular function, HuD is involved in processes such as neuronal commitment, neurite outgrowth, and axonal recovery after injury. It achieves these functions by stabilizing target mRNAs, enhancing their translation, and regulating alternative splicing and polyadenylation of neuronal transcripts. HuD's function also extends to the regulation of the senescence-associated secretory phenotype (SASP) in cells. Downregulation of HuD can lead to increased levels of SASP factors, sensitizing cells to senescence inducers and promoting cellular senescence.

免疫印迹
- WB result of HuD/ELAVL4 Recombinant Rabbit mAb
  Primary antibody: HuD/ELAVL4 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: SH-SY5Y whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 42 kDa
  Observed MW: 38 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) labelling HuD/ELAVL4 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- HuD/ELAVL4 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating HuD/ELAVL4 in 0.4 mg SH-SY5Y whole cell lysate.
  Western blot was performed on the immunoprecipitate using HuD/ELAVL4 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: SH-SY5Y whole cell lysate 20 µg (Input)
  Lane 2: HuD/ELAVL4 Rabbit mAb IP in SH-SY5Y whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in SH-SY5Y whole cell lysate
  Predicted MW: 42 kDa
  Observed MW: 38 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human hippocampus. Anti- HuD/ELAVL4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- HuD/ELAVL4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti- HuD/ELAVL4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in SH-SY5Y cells. Anti-HuD/ELAVL4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
- IF shows positive staining in paraffin-embedded human hippocampus. Anti- HuD/ELAVL4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.