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Rabbit抗原名称
Progranulin/PGRN分子别名
Acrogranin; Epithelin/granulin precursor; Glycoprotein of 88 Kda (GP88; Glycoprotein 88); PC cell-derived growth factor (PCDGF); Proepithelin (PEPI); Grn免疫原
Recombinant Protein细胞定位
Secreted, LysosomeAccession
P28798克隆号
S-1090-110抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms IP 1:50 Ms IHC-P 1:500 Ms, Rt ICC 1:500 Ms
Progranulin, also known as granulin-epithelin precursor (GEP), PC cell-derived growth factor (PCDGF), acrogranin, and proepithelin, is a secreted glycoprotein with a wide range of biological functions. In the context of neurodegenerative diseases, progranulin has emerged as a key factor. It is highly expressed in the brain and is implicated in the pathogenesis of frontotemporal lobar degeneration (FTLD), a type of dementia characterized by the accumulation of abnormal proteins in the brain's frontotemporal lobes. Mutations in the progranulin gene can lead to a decrease in its levels, which is associated with an increased risk of developing FTLD. Progranulin also plays a role in metabolic diseases. It has been identified as an adipokine that is involved in diet-induced obesity and insulin resistance, revealing its metabolic function. Moreover, progranulin has been linked to inflammation and immune responses. It can modulate inflammation by binding to tumor necrosis factor receptors (TNFRs) and potentially influencing TNF-dependent signaling.
免疫印迹
WB result of Progranulin/PGRN Recombinant Rabbit mAb
Primary antibody: Progranulin/PGRN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: J774A.1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 63 kDa
免疫沉淀
Progranulin/PGRN Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Progranulin/PGRN in 0.4 mg J774A.1 whole cell lysate.
Western blot was performed on the immunoprecipitate using Progranulin/PGRN Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: J774A.1 whole cell lysate 20 µg (Input)
Lane 2: Progranulin/PGRN Rabbit mAb IP in J774A.1 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in J774A.1 whole cell lysate
Predicted MW: 63 kDa
Observed MW: 63 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse pancreas. Anti- Progranulin/PGRN antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat pancreas. Anti- Progranulin/PGRN antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in J774A.1 cells. Anti-Progranulin/PGRN antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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