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Rabbit抗原名称
LEF1分子别名
Lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha (TCF1-alpha)免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q9UJU2克隆号
S-500-34抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
ChIP
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IP 1:50 Hu IHC-P 1:250 Hu, Ms, Rt ICC 1:500 Hu ICFCM 1:5000 Hu ChIP 1:20-1:50 Hu
LEF1 (Lymphoid Enhancer-Binding Factor 1) is a transcription factor belonging to the High-Mobility Group (HMG) family of proteins, which plays a role in various biological processes, including cell proliferation, differentiation, and survival. LEF1 is a key downstream effector of the Wnt/β-catenin signaling pathway and can regulate the transcription of target genes by binding with β-catenin. In cancer research, the abnormal expression of LEF1 is associated with the occurrence and progression of various types of cancer. It also plays a role in stem cell maintenance and organ development, particularly in the process of Epithelial-Mesenchymal Transition (EMT), where it activates the transcription of EMT effectors such as N-Cadherin, Vimentin, and Snail. In certain cancer cell types, such as Chronic Lymphocytic Leukemia (CLL), Burkitt's Lymphoma (BL), Acute Lymphoblastic Leukemia (ALL), Oral Squamous Cell Carcinoma (OSCC), and Colorectal Cancer (CRC), the activity of LEF1 makes it a valuable biomarker for predicting patient prognosis. Furthermore, LEF1 promotes the expression and activity of the androgen receptor in prostate cancer in an androgen-independent manner, ultimately increasing the growth of prostate cancer regardless of androgen ablation therapy. LEF1's inhibition or knockdown has been shown to slow down cancer growth, migration, and invasion, making it a potential target for cancer treatment. In the context of hematological malignancies, the expression and function of LEF1 differ between normal and leukemic hematopoiesis, and its expression in Chronic Lymphocytic Leukemia (CLL) is closely related to the progression and prognosis of the disease. The upregulation of LEF1 in CLL/Small Lymphocytic Lymphoma (SLL) may begin at an early stage of the disease.
免疫印迹
WB result of LEF1 Recombinant Rabbit mAb
Primary antibody: LEF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: LNCaP whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: Ramos whole cell lysate 20 µg
Negative control: LNCaP whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 30~55 kDaWB result of LEF1 Recombinant Rabbit mAb
Primary antibody: LEF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 30~55 kDaWB result of LEF1 Recombinant Rabbit mAb
Primary antibody: LEF1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat thymus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 30~55 kDa
流式分析
Flow cytometric analysis of LnCAP (Human prostate carcinoma epithelial cell, Left) / Jurkat (Human T cell leukemia T lymphocyte, right) labelling LEF1 antibody at 1/5000 dilution (0.01 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: LnCAP
免疫沉淀
LEF1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating LEF1 in 0.4 mg Jurkat whole cell lysate.
Western blot was performed on the immunoprecipitate using LEF1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate 20 µg (Input)
Lane 2: LEF1 Rabbit mAb IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
Predicted MW: 44 kDa
Observed MW: 30~55 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human thymus. Anti-LEF1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thymus. Anti-LEF1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-LEF1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-LEF1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Jurkat cells (top panel) and negative staining in LNcap cells (below panel). Anti-LEF1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on Jurkat cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used LEF1 Recombinant Rabbit mAb (S-500-34) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post -immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of AXIN2 and SAT-α in
LEF1 Recombinant Rabbit mAb (S-500-34)
-immunoprecipitated sample.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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