热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 其他一抗

所有图片（1/2）

Firefly Luciferase Rabbit Polyclonal Antibody

Luciferin 4-monooxygenase

价格 ￥400.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0975
规格
数量
收藏 分享
产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Firefly Luciferase

  • 分子别名

    Luciferin 4-monooxygenase

  • 免疫原

    Synthetic Peptide

  • Accession

    P08659

  • 抗体同种型

    IgG

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

    IP

  • 稀释度

    应用 稀释度
    WB 1:1000
    IP 1:50
背景介绍

  • Firefly Luciferase, also known as Photinus pyralis luciferase (FLuc), is a widely used enzyme in bioluminescence imaging due to its well-characterized properties and high sensitivity in detecting light output. The enzyme catalyzes the oxidation of luciferin in the presence of oxygen, ATP, and Mg2+ to produce a yellow-green light with a peak emission at 560-578 nm. This bioluminescent reaction is highly efficient and does not require any additional substrates or cofactors, making it an ideal reporter for gene expression studies and imaging in living organisms.

  • 免疫印迹

    • WB result of Firefly Luciferase Rabbit pAb
      Primary antibody: Firefly Luciferase Rabbit pAb at 1/1000 dilution
      Lane 1: untreated 293T whole cell lysate 5 µg
      Lane 2: 293T transfected with myc-His-Firefly Luciferase whole cell lysate 5 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 61 kDa
      Observed MW: 60 kDa

  • 免疫沉淀

    • Firefly Luciferase Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating Firefly Luciferase in 0.4 mg 293T transfected with myc-His-Firefly Luciferase whole cell lysate.
      Western blot was performed on the immunoprecipitate using Firefly Luciferase Rabbit pAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: 293T transfected with myc-His-Firefly Luciferase whole cell lysate 5 µg (Input)
      Lane 2: Firefly Luciferase Rabbit pAb IP in 293T transfected with myc-His-Firefly Luciferase whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in 293T transfected with myc-His-Firefly Luciferase whole cell lysate
      Predicted MW: 61 kDa
      Observed MW: 60 kDa

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

评论(0)