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Rabbit抗原名称
Cyclophilin B分子别名
Peptidyl-prolyl cis-trans isomerase B, PPIase B, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin (SCYLP), PPIB, CYPB免疫原
Synthetic Peptide细胞定位
Endoplasmic reticulumAccession
P23284抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Dg, Ck纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Ck, Dg IHC-P 1:200 Hu, Ms, Rt
Cyclophilin B, also known as CypB or PPIB, is an enzyme that belongs to the family of peptidyl-prolyl isomerases (PPIases). It plays a vital role in protein folding, a process that is essential for the proper functioning of proteins within the cell. Cyclophilin B is involved in various cellular processes, including the immune response, and has been implicated in the pathogenesis of several diseases, such as cancer and viral infections. In the context of viral infections, Cyclophilin B has been shown to interact with the hepatitis C virus (HCV) NS5B polymerase, enhancing its RNA-binding activity, which is crucial for viral replication. Inhibitors of cyclophilin B have demonstrated antiviral activity against HCV, both in vitro and in vivo, suggesting a potential therapeutic target for the treatment of HCV infection. Moreover, Cyclophilin B has been reported to be overexpressed in various cancers, including non-small cell lung carcinoma, hepatocellular carcinoma, and pancreatic carcinoma. Its overexpression has been correlated with reduced patient survival, indicating a possible role in tumor progression.
免疫印迹
WB result of Cyclophilin B Rabbit pAb
Primary antibody: Cyclophilin B Rabbit pAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Weak expression: Jurkat whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 17 kDaWB result of Cyclophilin B Rabbit pAb
Primary antibody: Cyclophilin B Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 17 kDaWB result of Cyclophilin B Rabbit pAb
Primary antibody: Cyclophilin B Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 17 kDaWB result of Cyclophilin B Rabbit pAb
Primary antibody: Cyclophilin B Rabbit pAb at 1/1000 dilution
Lane 1: dog liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 17 kDaWB result of Cyclophilin B Rabbit pAb
Primary antibody: Cyclophilin B Rabbit pAb at 1/1000 dilution
Lane 1: chicken liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 17 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti- Cyclophilin B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- Cyclophilin B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti- Cyclophilin B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- Cyclophilin B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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