CDK6 Recombinant Rabbit mAb (S-1312-23)

Cyclin-dependent kinase 6,Cell division protein kinase 6,Serine/threonine-protein kinase PLSTIRE,CDKN6

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0970

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

CDK6
分子别名

Cyclin-dependent kinase 6; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE; CDKN6
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q00534
克隆号

S-1312-23
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM ,
IHC-P ? ,
ICC ? ,
WB ,
IP
稀释度

应用稀释度

WB 1:4000
IP 1:50
IHC-P 1:500
ICC 1:2000
ICFCM 1:200

应用	稀释度
WB	1:4000
IP	1:50
IHC-P	1:500
ICC	1:2000
ICFCM	1:200

背景介绍

CDK6 (Cyclin-dependent kinase 6) is a protein kinase that belongs to the CDK family, which plays a significant role in the cell cycle. It is involved in driving the cell cycle from the G1 phase into the S phase by forming complexes with D-type cyclins (D1, D2, and D3). CDK6, along with CDK4, phosphorylates the retinoblastoma (Rb) protein family, leading to the release of E2F transcription factors and enabling G1 to S phase progression and DNA synthesis. CDK6 is not only important for cell cycle regulation but also has kinase-independent functions, such as acting as a transcriptional regulator. This dual functionality makes CDK6 an attractive target for therapeutic intervention in various types of cancer, including hematopoietic malignancies, breast cancer, and melanoma.

免疫印迹
- WB result of CDK6 Recombinant Rabbit mAb
  Primary antibody: CDK6 Recombinant Rabbit mAb at 1/4000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: K562 whole cell lysate 20 µg
  Lane 4: HEK-293 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 37 kDa
  Observed MW: 37 kDa
- WB result of CDK6 Recombinant Rabbit mAb
  Primary antibody: CDK6 Recombinant Rabbit mAb at 1/4000 dilution
  Lane 1: mouse spleen lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 37 kDa
  Observed MW: 37 kDa
- WB result of CDK6 Recombinant Rabbit mAb
  Primary antibody: CDK6 Recombinant Rabbit mAb at 1/4000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 37 kDa
  Observed MW: 37 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling CDK6 zeta antibody at 1/200 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- CDK6 Rabbit mAb at 1/200 dilution (1 µg) immunoprecipitating CDK6 in 0.4 mg K562 whole cell lysate.
  Western blot was performed on the immunoprecipitate using CDK6 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: K562 whole cell lysate 20 µg (Input)
  Lane 2: CDK6 Rabbit mAb IP in K562 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in K562 whole cell lysate
  Predicted MW: 37 kDa
  Observed MW: 37 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti- CDK6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human bladder cancer. Anti- CDK6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- CDK6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung cancer. Anti- CDK6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- CDK6 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-CDK6 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).