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sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb (S-807-137)

VEGFR-1,FRT

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0966
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    sFlt-1/VEGF Receptor 1

  • 分子别名

    VEGFR-1, FRT

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Secreted

  • Accession

    P17948

  • 克隆号

    S-807-137

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

    IP

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
    IP 1:50 Hu
背景介绍

  • Vascular endothelial growth factor (VEGF) represents a growth factor with important pro-angiogenic activity, having a mitogenic and an anti-apoptotic effect on endothelial cells, increasing the vascular permeability, promoting cell migration, etc. VEGF binds to tyrosine kinase cell receptors (VEGFRs): VEGFR-1 [Fms-like tyrosine kinase 1 (Flt-1)], VEGFR-2 [kinase insert domain receptor (KDR) in human; fetal liver kinase 1 (Flk-1) in mouse] and VEGFR-3 [Fms-like tyrosine kinase 4 (Flt-4)]. While VEGFR-1 and VEGFR-2 are expressed predominantly on vascular endothelial cells, VEGFR-3 is expressed especially on lymphatic endothelial cells.

  • 免疫印迹

    • WB result of sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb
      Primary antibody: sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HUVEC whole cell lysate 20 µg
      Lane 2: A431 whole cell lysate 20 µg
      Lane 3: Jurkat whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 150 kDa
      Observed MW: 180 kDa

    • WB result of sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb
      Primary antibody: sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse brain whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 150 kDa
      Observed MW: 180 kDa

  • 免疫沉淀

    • sFlt-1/VEGF Receptor 1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating sFlt-1/VEGF Receptor 1 in 0.4 mg A431 whole cell lysate.
      Western blot was performed on the immunoprecipitate using sFlt-1/VEGF Receptor 1 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: A431 whole cell lysate 10 µg (Input)
      Lane 2: sFlt-1/VEGF Receptor 1 Rabbit mAb IP in A431 whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in A431 whole cell lysate
      Predicted MW: 150 kDa
      Observed MW: 180 kDa

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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