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Rabbit抗原名称
Tuberin/TSC2分子别名
Tuberous sclerosis 2 protein免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P49815克隆号
S-1138-125抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB, IP反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IP | 1:50 |
| IHC-P | 1:200 |
Tuberin, the protein product of the TSC2 gene, is a tumor suppressor that plays a critical role in regulating cell growth, proliferation, and survival. It functions as a negative regulator in the mammalian target of rapamycin (mTOR) pathway, which controls cell growth in response to nutrients, growth factors, and energy status. Tuberin forms a complex with hamartin, another protein encoded by the TSC1 gene, and this complex functions to inhibit the GTPase activity of Rheb, a small G-protein that activates mTOR. Tuberin is sensitive to various cellular signals and can be modulated by a range of receptor tyrosine kinases (RTKs) and G protein-coupled receptors (GPCRs), indicating its role in integrating diverse cellular signals. It is also involved in other cellular processes such as transcriptional regulation, DNA repair, and endocytosis. Mutations or loss of function of tuberin lead to constitutive activation of the mTOR pathway, resulting in uncontrolled cell growth and proliferation, which is a hallmark of tuberous sclerosis complex (TSC), a genetic disorder characterized by the development of benign tumors in multiple organs.
免疫印迹
WB result of Tuberin/TSC2 Recombinant Rabbit mAb
Primary antibody: Tuberin/TSC2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 200 kDa
Observed MW: 200 kDaWB result of Tuberin/TSC2 Recombinant Rabbit mAb
Primary antibody: Tuberin/TSC2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 200 kDa
Observed MW: 200 kDaWB result of Tuberin/TSC2 Recombinant Rabbit mAb
Primary antibody: Tuberin/TSC2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 200 kDa
Observed MW: 200 kDa
免疫沉淀
Tuberin/TSC2 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Tuberin/TSC2 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using Tuberin/TSC2 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: Tuberin/TSC2 Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 200 kDa
Observed MW: 200 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human stomach. Anti- Tuberin/TSC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti- Tuberin/TSC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- Tuberin/TSC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti- Tuberin/TSC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti- Tuberin/TSC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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