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SMAD3 (phospho S423+S425) Recombinant Rabbit mAb (S-541-321)

Mothers against decapentaplegic homolog 3,MAD homolog 3,Mad3,Mothers against DPP homolog 3,hMAD-3,JV15-2,SMAD family member 3,MADH3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0948

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

SMAD3 (phospho S423+S425)
分子别名

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; MADH3
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P84022
克隆号

S-541-321
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Ms
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
ChIP	1:20-1:50	Hu

背景介绍

SMAD3 is a protein intracellular signal transducer that is part of the TGF-β (transforming growth factor-beta) superfamily signaling pathway. When SMAD3 is phosphorylated at serine residues 423 and 425 (referred to as SMAD3 (phospho S423+S425)), it indicates activation of the TGF-β signaling cascade within a cell. This phosphorylation event typically occurs after SMAD3 binds to the activated TGF-β receptor, leading to its phosphorylation and subsequent interaction with SMAD4. Together, they accumulate in the nucleus where they regulate gene expression associated with various cellular processes, including cell growth, differentiation, and immune responses. In the context of disease research, understanding SMAD3 phosphorylation is particularly relevant in conditions such as cancer, where dysregulation of TGF-β signaling can contribute to tumor progression and metastasis. SMAD3 is also implicated in fibrotic diseases, cardiovascular diseases, and immune responses, making it a potential therapeutic target for these conditions.

免疫印迹
- WB result of SMAD3 (phospho S423 + S425) Recombinant Rabbit mAb
  Primary antibody: SMAD3 (phospho S423 + S425) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HT-1080 whole cell lysate 20 µg
  Lane 2: HT-1080 treated with 10 ng/ml TGF-β for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 55 kDa
- WB result of SMAD3 (phospho S423 + S425) Recombinant Rabbit mAb
  Primary antibody: SMAD3 (phospho S423 + S425) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated C2C12 whole cell lysate 20 µg
  Lane 2: C2C12 treated with 10 ng/ml TGF-β for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 55 kDa
- WB result of SMAD3 (phospho S423 + S425) Recombinant Rabbit mAb
  Primary antibody: SMAD3 (phospho S423 + S425) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated C6 whole cell lysate 20 µg
  Lane 2: C6 treated with 10 ng/ml TGF-β for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 55 kDa
免疫沉淀
- Smad3 (phospho S423 + S425) Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Smad3 (phospho S423 + S425) in 0.4 mg HT-1080+TGF-β(10 ng/ml, 30 min) whole cell lysate.
  Western blot was performed on the immunoprecipitate using Smad3 (phospho S423 + S425) Recombinant Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HT-1080+TGF-β(10 ng/ml, 30 min) whole cell lysate 20 µg (Input)
  Lane 2: Smad3 (phospho S423 + S425) Recombinant Rabbit mAb IP in HT-1080+TGF-β(10 ng/ml, 30 min) whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HT-1080+TGF-β(10 ng/ml, 30 min) whole cell lysate
  Predicted MW: 48 kDa
  Observed MW: 55 kDa
  Exposure time: 20 s
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HT-1080+TGF-β (10 ng/ml, 30 min) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used SMAD3 (phospho S423+S425) Recombinant Rabbit mAb (S-541-321) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post -immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of CDKN1A, ID1 and SAT-α in SMAD3 (phospho S423
  +S425) Recombinant Rabbit mAb (S-541-321)-immunoprecipitated sample.