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M6PR Recombinant Rabbit mAb (S-1480-21)

Cation-independent mannose-6-phosphate receptor,CI Man-6-P receptor,CI-MPR,300 kDa mannose 6-phosphate receptor (MPR 300),Insulin-like growth factor 2 receptor,Insulin-like growth factor II receptor (IGF-II receptor),M6P/IGF2 receptor (M6P/IGF2R),CD222,IGF2R,MPRI

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0944

规格

25μl100μl1ml

数量

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

M6PR
分子别名

Cation-independent mannose-6-phosphate receptor, CI Man-6-P receptor, CI-MPR, 300 kDa mannose 6-phosphate receptor (MPR 300), Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor (IGF-II receptor), M6P/IGF2 receptor (M6P/IGF2R), CD222, IGF2R, MPRI
免疫原

Synthetic Peptide
细胞定位

Golgi apparatus membrane
Accession

P11717
克隆号

S-1480-21
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM ,
IHC-P ? ,
ICC ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:200 Hu, Ms, Rt
ICC 1:500 Hu, Ms
ICFCM 1:50 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:200	Hu, Ms, Rt
ICC	1:500	Hu, Ms
ICFCM	1:50	Hu, Ms

背景介绍

M6PR, or mannose 6-phosphate receptor, is a transmembrane glycoprotein that plays a critical role in cellular processes, particularly in the targeting of lysosomal enzymes. There are two types of M6PR: cation-dependent M6PR (CD-M6PR) and cation-independent M6PR (CI-M6PR). CI-M6PR is of particular interest due to its role in endocytosis and its potential as a therapeutic target. CI-M6PR is involved in the transport of newly synthesized lysosomal enzymes from the trans-Golgi network (TGN) to lysosomes, a process essential for cellular metabolism and catabolism homeostasis. It also mediates the endocytosis of extracellular ligands that bear M6P residues, such as insulin-like growth factor II (IGF-II) and retinoic acid, in addition to lysosomal enzymes. CI-M6PR's role extends beyond lysosomal enzyme targeting, as it has been implicated in cancer, immunology, and brain function. In cancer, CI-M6PR is overexpressed in several types of cancer cells, suggesting its potential as a target for cancer therapy. In the immune system, it plays a role in cell migration, wound healing, and viral infection.

免疫印迹
- WB result of M6PK Recombinant Rabbit mAb
  Primary antibody: M6PK Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: A549 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Weak expression: A549 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 274 kDa
  Observed MW: 280 kDa
- WB result of M6PK Recombinant Rabbit mAb
  Primary antibody: M6PK Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 274 kDa
  Observed MW: 280 kDa
- WB result of M6PK Recombinant Rabbit mAb
  Primary antibody: M6PK Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 274 kDa
  Observed MW: 280 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling M6PR antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling M6PR antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-M6PR antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-M6PR antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-M6PR antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-M6PR antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-M6PR antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-M6PR antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-M6PR antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti-M6PR antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-M6PR antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti-M6PR antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).