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KAP-1 Recombinant Rabbit mAb (S-986-5)

Transcription intermediary factor 1-beta,TIF1-beta,E3 SUMO-protein ligase TRIM28,KRAB-associated protein 1 (KAP-1),KRAB-interacting protein 1 (KRIP-1),Nuclear corepressor KAP-1,RING finger protein 96,RING-type E3 ubiquitin transferase TIF1-beta,Tripartite motif-containing protein 28,TRIM28,RNF96,TIF1B

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0943

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

KAP-1
分子别名

Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1 (KAP-1), KRAB-interacting protein 1 (KRIP-1), Nuclear corepressor KAP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, Tripartite motif-containing protein 28, TRIM28, RNF96, TIF1B
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

Q13263
克隆号

S-986-5
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IP 1:50
IHC-P 1:1000
ICC 1:500
ICFCM 1:500

应用	稀释度
WB	1:1000
IP	1:50
IHC-P	1:1000
ICC	1:500
ICFCM	1:500

背景介绍

KAP-1, also known as KRAB-associated protein 1 or TIF1β (transcriptional intermediary factor 1β), is a multifunctional protein involved in a variety of cellular processes, including transcriptional regulation, DNA repair, and maintenance of genomic integrity. KAP-1 functions as a transcriptional corepressor by interacting with KRAB-ZNFs, which recruit it to specific genomic loci. Once bound, KAP-1 can influence the epigenetic state of the targeted DNA, leading to transcriptional repression through the recruitment of chromatin-modifying enzymes such as histone deacetylases (HDACs) and histone methyltransferases (HMTs) like SETDB1. This results in the formation of repressive chromatin marks, such as H3K9me3, which contribute to the establishment of a heterochromatic environment. In addition to its role in transcriptional regulation, KAP-1 has been implicated in the maintenance of pluripotency in stem cells and is required for terminal differentiation of mouse embryonic stem cells. It is also associated with promoting and inhibiting differentiation of various adult cell types, suggesting a complex role in cellular development and differentiation.

免疫印迹
- WB result of KAP-1 Recombinant Rabbit mAb
  Primary antibody: KAP-1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: A431 whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 89 kDa
  Observed MW: 105 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling KAP-1 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- KAP-1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating KAP-1 in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using KAP-1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: KAP-1 Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 89 kDa
  Observed MW: 105 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti- KAP-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti- KAP-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti- KAP-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti- KAP-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-KAP-1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).