Histone H4 (acetyl K5) Recombinant Rabbit mAb (S-1464-112)

H4K5ac

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0942

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Histone H4 (acetyl K5)
分子别名

H4K5ac
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P62805
克隆号

S-1464-112
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

乙酰化
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Fs, Fu, Pl, Ys, Ck
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:2000 Hu, Ms
IP 1:50 Ms
IHC-P 1:500-1:2000 Hu, Ms, Rt

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:2000	Hu, Ms
IP	1:50	Ms
IHC-P	1:500-1:2000	Hu, Ms, Rt

背景介绍

Histone H4 acetylation at lysine 5 (H4K5ac) is a post-translational modification that plays a significant role in chromatin structure and function. This modification is catalyzed by histone acetyltransferases (HATs), which transfer an acetyl group from acetyl-coenzyme A (acetyl-CoA) to the ε-amino group of the lysine residue on histone H4. The acetylation of histone H4 at lysine 5 is crucial for various cellular processes, including gene activation, DNA replication, and cell cycle progression. It is involved in providing a more open chromatin structure, which is generally permissive for the binding of transcription factors and the assembly of the transcription machinery. Additionally, H4K5ac has been linked to the deposition of the histone H3 variant CENP-A into centromeres, which is essential for proper chromosome segregation during cell division.

免疫印迹
- WB result of Histone H4 (acetyl K5) Recombinant Rabbit mAb
  Primary antibody: Histone H4 (acetyl K5) Recombinant Rabbit mAb at 1/2000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 11 kDa
  Observed MW: 11 kDa
- WB result of Histone H4 (acetyl K5) Recombinant Rabbit mAb
  Primary antibody: Histone H4 (acetyl K5) Recombinant Rabbit mAb at 1/2000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 11 kDa
  Observed MW: 11 kDa
免疫沉淀
- Histone H4 (acetyl K5) Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating Histone H4 (acetyl K5) in 0.4 mg NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate.
  Western blot was performed on the immunoprecipitate using Histone H4 (acetyl K5) Rabbit pAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 5 µg (Input)
  Lane 2: Histone H4 (acetyl K5) Rabbit pAb IP in NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate
  Predicted MW: 11 kDa
  Observed MW: 11 kDa
斑点杂交
- Dot blot result of Histone H4 (acetyl K5) Recombinant Rabbit mAb
  Lane 1: H4K5ac modified peptide
  Lane 2: H4K5 unmodified peptide
  Lane 3: H2AK5ac modified peptide
  Primary antibody: Histone H4 (acetyl K5) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti- Histone H4 (acetyl K5) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti- Histone H4 (acetyl K5) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human testis. Anti- Histone H4 (acetyl K5) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse lung. Anti- Histone H4 (acetyl K5) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti- Histone H4 (acetyl K5) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat colon. Anti- Histone H4 (acetyl K5) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti- Histone H4 (acetyl K5) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.