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宿主来源
Rabbit抗原名称
E-selectin分子别名
CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1 (ELAM-1), Leukocyte-endothelial cell adhesion molecule 2 (LECAM2), CD62E, SELE, ELAM1免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P16581克隆号
S-1260-26抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:500 |
E-selectin, also known as CD62E, is a cell adhesion molecule that plays a significant role in the immune system, particularly during inflammation and the recruitment of leukocytes to sites of infection or tissue damage. It is expressed on the surface of endothelial cells and facilitates the rolling and adhesion of leukocytes, such as neutrophils and monocytes, along the endothelium. E-selectin's interaction with its ligands on leukocytes is crucial for the initiation of inflammatory responses. It binds to specific carbohydrate structures present on the surface of leukocytes, mediating the initial steps of leukocyte extravasation into tissues. This process is highly regulated and involves a dynamic expression of E-selectin, which is upregulated in response to inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β). In addition to its role in leukocyte recruitment, E-selectin has been implicated in the activation of cellular processes within neutrophils.
免疫印迹
WB result of E-selectin Recombinant Rabbit mAb
Primary antibody: E-selectin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HUVEC whole cell lysate 20 µg
Lane 2: HUVEC treated with 25 ng/ml TNF-alpha for 6 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 90~120 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti- E-selectin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- E-selectin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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