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Rabbit抗原名称
AACT分子别名
Alpha-1-antichymotrypsin, ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3免疫原
Recombinant Protein细胞定位
SecretedAccession
P01011克隆号
S-897-32抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
IP
稀释度
应用 稀释度 WB 1:1000 IP 1:50
Alpha-1-antichymotrypsin (AACT), also known as SERPIN family A member 3 (SERPINA3), is a glycoprotein primarily synthesized in the liver and astrocytes, then secreted into surrounding tissues or blood, where it plays significant roles. AACT is capable of binding to mast cell chymase and neutrophil cathepsin G, thereby protecting cells and tissues from damage. In various conditions such as fibromyalgia, multiple sclerosis, chronic heart failure, Alzheimer's disease, and certain cancers, AACT levels are elevated and can serve as a diagnostic or prognostic biomarker.
免疫印迹
WB result of AACT Recombinant Rabbit mAb
Primary antibody: AACT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: human serum lysate 5 µg
Lane 2: human plasma lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 60~70 kDa
免疫沉淀
AACT Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating AACT in 0.4 mg human serum lysate.
Western blot was performed on the immunoprecipitate using AACT Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: human serum lysate 5 µg (Input)
Lane 2: AACT Rabbit mAb IP in human serum lysate
Lane 3: Rabbit monoclonal IgG IP in human serum lysate
Predicted MW: 48 kDa
Observed MW: 60~70 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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