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宿主来源
Rat克隆号
S-R483抗体类型
Rat mAb抗体同种型
Rat IgG2a, κ纯化方式
Protein G浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<2EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
应用
In vivo control
IHC-P ?
FCM
WB
稀释度
应用 稀释度 WB 1:1000 IHC-P 1:500 FCM 1:500
Isotype control antibodies, to estimate the nonspecific binding of target.
免疫印迹
WB result of Invivo rat IgG2a isotype control, anti-trinitrophenol
Primary antibody: Invivo rat IgG2a isotype control, anti-trinitrophenol at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilutionWB result of Invivo rat IgG2a isotype control, anti-trinitrophenol
Primary antibody: Invivo rat IgG2a isotype control, anti-trinitrophenol at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilutionWB result of Invivo rat IgG2a isotype control, anti-trinitrophenol
Primary antibody: Invivo rat IgG2a isotype control, anti-trinitrophenol at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling rat IgG2a isotype control antibody at 1/500 dilution (1 μg) / (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows negative staining in paraffin-embedded mouse kidney. rat IgG2a isotype control was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded mouse spleen. rat IgG2a isotype control was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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