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Rabbit抗原名称
LRP1分子别名
Prolow-density lipoprotein receptor-related protein 1, LRP-1, Alpha-2-macroglobulin receptor (A2MR), Apolipoprotein E receptor (APOER), A2MR, APR免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cytoskeleton, Cell membraneAccession
Q07954抗体同种型
IgG反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Ck纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IP 1:50 Hu IHC-P 1:200 Hu, Ms, Rt
Low-density lipoprotein receptor-related protein 1 (LRP1) is a critical endocytic receptor involved in the transport and internalization of various ligands, including lipoproteins, proteases, and cytokines, playing a significant role in lipid metabolism, inflammation, and tissue repair. It is also implicated in the pathogenesis of Alzheimer's disease by regulating the uptake and spread of tau protein, and has been identified as a potential target for enhancing drug delivery across the blood-labyrinth barrier in the inner ear, offering a promising avenue for the treatment of hearing disorders.
免疫印迹
WB result of LRP1 Rabbit pAb
Primary antibody: LRP1 Rabbit pAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 85 kDa
Observed MW: 90 kDaWB result of LRP1 Rabbit pAb
Primary antibody: LRP1 Rabbit pAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 85 kDa
Observed MW: 90 kDaWB result of LRP1 Rabbit pAb
Primary antibody: LRP1 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 85 kDa
Observed MW: 90 kDa
免疫沉淀
LRP1 Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating LRP1 in 0.4 mg U-87 MG whole cell lysate.
Western blot was performed on the immunoprecipitate using LRP1 Rabbit pAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: U-87 MG whole cell lysate 20 µg (Input)
Lane 2: LRP1 Rabbit pAb IP in U-87 MG whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in U-87 MG whole cell lysate
Predicted MW: 85 kDa
Observed MW: 90 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-LRP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-LRP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-LRP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-LRP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-LRP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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