大包装询价 产品介绍 评论(0)
宿主来源
Mouse抗原名称
CD28分子别名
T-cell-specific surface glycoprotein CD28, TP44细胞定位
Cell membraneAccession
P10747克隆号
S-R495-2抗体类型
Mouse mAb抗体同种型
IgG应用
FCM反应种属 ?
Hu纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| FCM | 1:2000 |
CD28 is a key costimulatory receptor predominantly expressed on T cells, which plays an essential role in T cell activation, proliferation, and survival. Upon binding to its ligands CD80 (B7-1) and CD86 (B7-2) expressed on antigen-presenting cells, CD28 provides a secondary signal that amplifies the initial T cell receptor engagement with the antigen. This costimulation is crucial for preventing T cell anergy and for the full execution of immune responses, including the production of cytokines and the development of memory T cells. CD28 also contributes to the induction and maintenance of regulatory T cells, and its signaling involves the activation of multiple downstream pathways, including the PI3K-AKT axis, which is important for cell metabolism and survival.
流式分析
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD40 antibody at 1/2000 (0.1 μg) dilution (Right) compared with a Mouse monoclonal IgG isotype control (Left). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Brilliant Violet 421™ separately. Events were gated on viable lymphocytes.
评论(0)