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Rabbit抗原名称
b-FGF/FGF2分子别名
Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor (bFGF), Heparin-binding growth factor 2 (HBGF-2), FGFB免疫原
Synthetic Peptide细胞定位
Secreted, NucleusAccession
P09038克隆号
S-1161-136抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu预测反应种属
(反应种属缩写表)Cz, Bv, Rb纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 WB 1:1000 IHC-P 1:250-1:1000 ICC 1:500 ICFCM 1:500
b-FGF, also known as FGF-2, is a potent mitogenic protein belonging to the fibroblast growth factor family. It plays a crucial role in cell proliferation, differentiation, and survival. b-FGF is particularly important for angiogenesis, wound healing, and tissue repair. It binds with high affinity to heparin and specifically to tyrosine kinase receptors, activating the FGF/FGFR signaling pathway, which in turn influences various cellular processes. b-FGF is widely distributed across different tissues, with highest concentrations found in the pituitary, brain, and neural tissues, retina, adrenal glands, and placenta. In cell culture, b-FGF is an essential growth factor for maintaining the undifferentiated state of stem cells, promoting the proliferation and differentiation of neural cells, and extending the survival of neurons. It also significantly promotes the proliferation and migration of endothelial cells when used in conjunction with heparin. The broad biological activities of b-FGF make it a key factor in biomedical research and clinical applications, particularly in regenerative medicine and the development of therapeutic strategies for various diseases.
免疫印迹
WB result of b-FGF/FGF2 Recombinant Rabbit mAb
Primary antibody: b-FGF/FGF2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Lane 4: SK-OV-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 15, 22, 24 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized SK-OV-3 (Human ovarian cancer epithelial cell) labelling b-FGF/FGF2 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- b-FGF/FGF2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti- b-FGF/FGF2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti- b-FGF/FGF2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti- b-FGF/FGF2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti- b-FGF/FGF2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti- b-FGF/FGF2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti- b-FGF/FGF2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in SK-OV-3 cells. Anti- b-FGF/FGF2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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