GHR Recombinant Rabbit mAb (S-1320-123)

Growth hormone receptor,GH receptor,Somatotropin receptor

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0917

规格

25μl100μl1ml

数量

产品介绍

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产品规格

应用	稀释度
WB	1:1000
ICC	1:50

背景介绍

The growth hormone receptor (GHR) is a cell surface receptor that binds to growth hormone (GH), a hormone responsible for stimulating growth, cell reproduction, and cell regeneration in humans. When GH binds to GHR, it triggers a cascade of intracellular signaling events, including the activation of JAK2/STAT, MAPK, and PI3K pathways, which play crucial roles in growth and development. GHR is highly expressed in liver cells but also found in other tissues such as the kidneys, skeletal muscle, and the central nervous system. Mutations or alterations in the GHR can lead to growth abnormalities and have been implicated in conditions like Laron syndrome, which is characterized by dwarfism and high levels of GH due to GHR insensitivity. Understanding the structure and function of GHR is vital for developing therapeutic strategies for growth-related disorders.

免疫印迹
- WB result of GHR Recombinant Rabbit mAb
  Primary antibody: GHR Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: A549 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 72 kDa
  Observed MW: 71 kDa
  This blot was developed with high sensitivity substrate
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti-GHR antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).