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Rabbit抗原名称
DAZL分子别名
Deleted in azoospermia-like; DAZ-like autosomal; Deleted in azoospermia-like 1; Dazl1; Dazla免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
Q64368抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IP
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IP 1:50 Ms IHC-P 1:250 Hu, Ms, Rt IF 1:250 Ms
DAZL, or Deleted in Azoospermia-Like, is an RNA-binding protein that plays a pivotal role in germ cell development and oocyte maturation. It is specifically expressed in germ cells and is essential for their proper differentiation and function. DAZL has been shown to regulate the translation of a variety of mRNAs, thereby controlling the expression of proteins critical for germ cell development. Recent studies have revealed that DAZL can function as both a repressor and activator of mRNA translation, depending on the context and the specific mRNA targets. It interacts with the 3' untranslated regions (UTRs) of its target mRNAs, modulating their stability and translation efficiency. DAZL's dual role in translational control is significant for understanding the molecular mechanisms underlying germ cell maturation and early embryonic development. The protein's function is so critical that its absence or mutation can lead to infertility, as seen in certain cases of male azoospermia.
免疫印迹
WB result of DAZL Rabbit pAb
Primary antibody: DAZL Rabbit pAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 38 kDaWB result of DAZL Rabbit pAb
Primary antibody: DAZL Rabbit pAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 38 kDa
免疫沉淀
DAZL Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating DAZL in 0.4 mg mouse testis lysate.
Western blot was performed on the immunoprecipitate using DAZL Rabbit pAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: mouse testis lysate 20 µg (Input)
Lane 2: DAZL Rabbit pAb IP in mouse testis lysate
Lane 3: Rabbit monoclonal IgG IP in mouse testis lysate
Predicted MW: 33 kDa
Observed MW: 38 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-DAZL antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-DAZL antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-DAZL antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse testis. Anti-DAZL antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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