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宿主来源
Rat抗原名称
CD45分子别名
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), T200, PTPRC, Lymphocyte antigen 5 (Ly-5)细胞定位
Cell membraneAccession
P06800克隆号
S-R477抗体类型
Recombinant mAb抗体同种型
IgG2b,k应用
FCM反应种属 ?
Ms纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| FCM | 1:2000 |
CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes via its extracellular domain (a form of co-stimulation), or by activating various Src family kinases required for the antigen receptor signaling via its cytoplasmic domain. CD45 also suppresses JAK kinases, and so functions as a negative regulator of cytokine receptor signaling.
流式分析
Flow cytometric analysis of mouse primary splenocytes labelling CD45 antibody at 1/2000 dilution (0.1 μg)/ (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Gated on total viable cells.
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