BMI-1 Recombinant Rabbit mAb (S-1075-252)

Polycomb complex protein BMI-1,Polycomb group RING finger protein 4,RING finger protein 51,PCGF4,RNF51

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0908

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

BMI-1
分子别名

Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, PCGF4, RNF51
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

P35226
克隆号

S-1075-252
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Ct, Bv, Ys
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ChIP

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu, Ms
ICFCM 1:50 Hu, Ms
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu, Ms
ICFCM	1:50	Hu, Ms
ChIP	1:20-1:50	Hu

背景介绍

Polycomb complex protein BMI-1, also known as B-cell specific Moloney murine leukemia virus integration site 1, is a component of the Polycomb repressive complex 1 (PRC1) that plays a significant role in gene silencing by regulating chromatin structure. BMI-1 is crucial for the self-renewal of both normal and cancer stem cells and is associated with the regulation of cell proliferation and senescence through the ink4a locus. It has been identified as an oncogene that can induce B- and T-cell leukemias and is frequently overexpressed in various types of cancer, including hematologic and solid cancers, suggesting its potential as a therapeutic target. BMI-1's involvement in cancer is not limited to its role in cell proliferation. It has also been shown to promote invasion and metastasis of cancer stem cells, particularly in pancreatic cancer, by activating the PI3K/AKT signaling pathway.

免疫印迹
- WB result of BMI-1 Recombinant Rabbit mAb
  Primary antibody: BMI-1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: A549 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 37 kDa
  Observed MW: 39 kDa
- WB result of BMI-1 Recombinant Rabbit mAb
  Primary antibody: BMI-1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 37 kDa
  Observed MW: 39 kDa
- WB result of BMI-1 Recombinant Rabbit mAb
  Primary antibody: BMI-1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 37 kDa
  Observed MW: 40 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling BMI-1 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling BMI-1 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-BMI-1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti-BMI-1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross -
  linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used BMI-1 Recombinant Rabbit mAb (S-1075-252) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  
  qPCR showed the enrichment of HOXA2 and SAT-α in BMI-1 Recombinant
  Rabbit mAb (S-1075-252)-immunoprecipitated sample.