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Rabbit抗原名称
Phospho-TAK1 (Ser439)分子别名
Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1 (TGF-beta-activated kinase 1); MAP3K7免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
O43318克隆号
S-1136-46抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu预测反应种属
(反应种属缩写表)Rt, Or, Ms, Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
IP
稀释度
应用 稀释度 WB 1:1000 IP 1:50
Phospho-TAK1 (Ser439) is a significant biomarker for the activation of TAK1 (Transforming growth factor-β activated kinase 1), which is a mitogen-activated protein kinase kinase kinase (MAP3K) involved in various cellular signaling pathways, including the MAPK and NF-κB pathways. The phosphorylation of TAK1 at serine 439 (or 412 in rodents) is a key event in its activation process. This modification allows TAK1 to interact with other proteins and initiate downstream signaling cascades that regulate cell growth, survival, and inflammation. The detection of Phospho-TAK1 (Ser439) is typically performed using specific antibodies that recognize this phosphorylated form of the protein.
免疫印迹
WB result of Phospho-TAK1 (Ser439) Recombinant Rabbit mAb
Primary antibody: Phospho-TAK1 (Ser439) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 nM Calyculin A and 20 ng/ml human IL-1β for 10 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 75 kDa
免疫沉淀
Phospho-TAK1 (Ser439) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-TAK1 (Ser439) in 0.4 mg HeLa treated with 100 nM Calyculin A and 20 ng/ml human IL-1β for 10 minutes whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-TAK1 (Ser439) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa treated with 100 nM Calyculin A and 20 ng/ml human IL-1β for 10 minutes whole cell lysate 20 µg (Input)
Lane 2: Phospho-TAK1 (Ser439) Rabbit mAb IP in HeLa treated with 100 nM Calyculin A and 20 ng/ml human IL-1β for 10 minutes whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa treated with 100 nM Calyculin A and 20 ng/ml human IL-1β for 10 minutes whole cell lysate
Predicted MW: 67 kDa
Observed MW: 75 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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