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宿主来源
Rabbit抗原名称
Phospho-TAK1 (Ser439)分子别名
Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1 (TGF-beta-activated kinase 1); MAP3K7免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
O43318克隆号
S-1136-46抗体类型
Recombinant mAb抗体同种型
IgG应用
WB, IP反应种属 ?
Hu预测反应种属
(反应种属缩写表)Rt, Or, Ms, Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IP | 1:50 |
Phospho-TAK1 (Ser439) is a significant biomarker for the activation of TAK1 (Transforming growth factor-β activated kinase 1), which is a mitogen-activated protein kinase kinase kinase (MAP3K) involved in various cellular signaling pathways, including the MAPK and NF-κB pathways. The phosphorylation of TAK1 at serine 439 (or 412 in rodents) is a key event in its activation process. This modification allows TAK1 to interact with other proteins and initiate downstream signaling cascades that regulate cell growth, survival, and inflammation. The detection of Phospho-TAK1 (Ser439) is typically performed using specific antibodies that recognize this phosphorylated form of the protein.
免疫印迹
WB result of Phospho-TAK1 (Ser439) Recombinant Rabbit mAb
Primary antibody: Phospho-TAK1 (Ser439) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 nM Calyculin A and 20 ng/ml human IL-1β for 10 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 75 kDa
免疫沉淀
Phospho-TAK1 (Ser439) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-TAK1 (Ser439) in 0.4 mg HeLa treated with 100 nM Calyculin A and 20 ng/ml human IL-1β for 10 minutes whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-TAK1 (Ser439) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa treated with 100 nM Calyculin A and 20 ng/ml human IL-1β for 10 minutes whole cell lysate 20 µg (Input)
Lane 2: Phospho-TAK1 (Ser439) Rabbit mAb IP in HeLa treated with 100 nM Calyculin A and 20 ng/ml human IL-1β for 10 minutes whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa treated with 100 nM Calyculin A and 20 ng/ml human IL-1β for 10 minutes whole cell lysate
Predicted MW: 67 kDa
Observed MW: 75 kDa
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