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Phospho-Histone H2A.X (Ser139) Recombinant Rabbit mAb (S-1368-15)

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0901
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-Histone H2A.X (Ser139)

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P16104

  • 克隆号

    S-1368-15

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ? ,
    WB ,
    IP

  • 稀释度

    应用 稀释度 推荐种属
    Dot Blot 1:1000
    WB 1:1000 Hu
    IP 1:50 Hu
    IHC-P 1:200-1:500 Hu, Ms, Rt
背景介绍

  • Phospho-Histone H2A.X (Ser139), also known as γH2A.X, is a variant of the histone H2A protein that plays a crucial role in the cellular response to DNA double-strand breaks (DSBs). When DNA damage occurs, such as that induced by ionizing radiation or anticancer drugs, the protein is rapidly phosphorylated at Ser139 by kinases like ATM, ATR, and DNA-PK. This phosphorylation event is one of the earliest responses to DNA damage and is essential for the recruitment of various DNA repair proteins to the site of the break.

  • 免疫印迹

    • WB result of Phospho-Histone H2A.X (Ser139) Recombinant Rabbit mAb
      Primary antibody: Phospho-Histone H2A.X (Ser139) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hour whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

  • 免疫沉淀

    • Phospho-Histone H2A.X (Ser139) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Histone H2A.X (Ser139) in 0.4 mg HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hour whole cell lysate.
      Western blot was performed on the immunoprecipitate using Phospho-Histone H2A.X (Ser139) Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hour whole cell lysate 20 µg (Input)
      Lane 2: Phospho-Histone H2A.X (Ser139) Rabbit mAb IP in HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hour whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hour whole cell lysate
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

  • 斑点杂交

    • Dot blot result of Phospho-Histone H2A.X (Ser139) Recombinant Rabbit mAb
      Lane 1: Histone H2A.X (Ser139) phospho peptide
      Lane 2: Histone H2A.X unmodified peptide
      Primary antibody: Phospho-Histone H2A.X (Ser139) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- Phospho-Histone H2A.X (Ser139) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human testis. Anti- Phospho-Histone H2A.X (Ser139) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- Phospho-Histone H2A.X (Ser139) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti- Phospho-Histone H2A.X (Ser139) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse testis. Anti- Phospho-Histone H2A.X (Ser139) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat testis. Anti- Phospho-Histone H2A.X (Ser139) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

检测磷酸化蛋白时，推荐有什么封闭体系？

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高

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