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TIM-3 Recombinant Rabbit mAb (S-1450-21)

Hepatitis A virus cellular receptor 2,HAVcr-2,T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3),T-cell immunoglobulin mucin receptor 3 (TIM-3),T-cell membrane protein 3,CD366,HAVCR2,TIMD3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0900

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

TIM-3
分子别名

Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3); T-cell immunoglobulin mucin receptor 3 (TIM-3); T-cell membrane protein 3; CD366; HAVCR2; TIMD3
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

Q8TDQ0
克隆号

S-1450-21
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
WB ,
IP ,
IF ?
稀释度

应用稀释度

WB 1:1000
IP 1:50
IHC-P 1:500
IF 1:200

应用	稀释度
WB	1:1000
IP	1:50
IHC-P	1:500
IF	1:200

背景介绍

TIM-3, also known as Hepatitis A virus Cell receptor 2 (HAVCR2), is a transmembrane protein and a member of the TIM protein family, which plays a crucial role in immune response regulation. It is expressed on various immune cells, including CD8+ T cells, Th1 cells, regulatory T cells (Tregs), dendritic cells (DCs), natural killer (NK) cells, monocytes, and macrophages. TIM-3 functions as an inhibitory receptor and is associated with T cell exhaustion in chronic infections and cancer. It has been identified as a promising target for cancer immunotherapy, as its blockade can enhance antitumor immunity. The receptor interacts with several ligands, including Galectin-9, CEACAM-1, phosphatidylserine (PtdSer), and High Mobility Group Box 1 (HMGB1). TIM-3 also plays a role in the clearance of apoptotic bodies by interacting with PtdSer on the surface of apoptotic cells. Recent studies have shown that targeting TIM-3 on DCs might be more critical for enhancing antitumor immunity than targeting it on T cells. Furthermore, combined blockade of TIM-3 and PD-1 has shown synergistic effects in reducing tumor growth in various mouse tumor models, suggesting that the combination therapy might be a promising approach for cancer treatment.

免疫印迹
- WB result of TIM-3 Recombinant Rabbit mAb
  Primary antibody: TIM-3 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: HT-29 whole cell lysate 20 µg
  Lane 3: Daudi whole cell lysate 20 µg
  Negative control: Jurkat whole cell lysate; HT-29 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 33 kDa
  Observed MW: 55 kDa
免疫沉淀
- TIM-3 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating TIM-3 in 0.4 mg Daudi whole cell lysate.
  Western blot was performed on the immunoprecipitate using TIM-3 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: Daudi whole cell lysate 20 µg (Input)
  Lane 2: TIM-3 Rabbit mAb IP in Daudi whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Daudi whole cell lysate
  Predicted MW: 33 kDa
  Observed MW: 55 kDa
  This blot was developed with high sensitivity substrate
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti- TIM-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human liver. Anti- TIM-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach. Anti- TIM-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti- TIM-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti- TIM-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- TIM-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
免疫荧光
- IF shows positive staining in paraffin-embedded human tonsil. Anti-TIM-3 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded human colon cancer. Anti-TIM-3 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.