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Rabbit抗原名称
Phospho-PRAS40 (T246)分子别名
Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate; AKT1S1免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
Q96B36克隆号
S-977-7抗体类型
Recombinant mAb抗体同种型
IgG应用
ICC, WB反应种属 ?
Hu预测反应种属
(反应种属缩写表)Ms纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| Dot Blot | 1:1000 |
| WB | 1:1000 |
| ICC | 1:500 |
PRAS40, which stands for Proline-rich Akt Substrate of 40 kDa, is a multifunctional protein that plays various roles within the cell, especially in biological processes such as cell growth, apoptosis, oxidative stress, autophagy, and angiogenesis. PRAS40 is a substrate of the protein kinase B (PKB/Akt) and also a binding protein for 14-3-3 proteins. It is expressed in a variety of tissues and has multiple phosphorylation sites, with its activity closely related to its phosphorylation status. PRAS40 is involved in several signaling pathways, including the mammalian target of rapamycin (mTOR) pathway, the protein kinase B (PKB/Akt) pathway, nuclear factor kappa-B (NF-κB), proto-oncogene serine/threonine-protein kinase PIM-1 (PIM1), and pyruvate kinase M2 (PKM2). PRAS40 plays a significant role in a variety of human diseases, including cardiovascular diseases, ischemia-reperfusion injury, neurodegenerative diseases, cancer, diabetes, and other metabolic diseases. Moreover, the nuclear localization of PRAS40 is associated with the Akt/mTORC1 signaling axis and the RPL11-HDM2-p53 nucleolar stress response pathway. The phosphorylation of PRAS40, especially at the Thr246 site, mediated by Akt and mTORC1, may have an important impact on mTORC1 activity.
免疫印迹
WB result of Phospho-PRAS40 (T246) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-PRAS40 (T246) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa starve overnight, then treated with 100 nM insulin for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 38 kDa
This blot was developed with high sensitivity substrate
斑点杂交
Dot blot result of Phospho-PRAS40 (T246) Recombinant Rabbit mAb
Lane1: Phospho-PRAS40 (T246) peptide
Lane2: PRAS40 unmodified peptide
Primary antibody: Phospho-PRAS40 (T246) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
ICC analysis of HeLa cells starve overnight, then treated with insulin (100nM) for 10 minutes (top panel) and untreated HeLa cells (below panel). Anti- Phospho-PRAS40 (T246) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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