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Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb (S-617-138)

Mitogen-activated protein kinase 14,MAP kinase 14,MAPK 14,Cytokine suppressive anti-inflammatory drug-binding protein (CSAID-binding protein,CSBP),MAP kinase MXI2,MAX-interacting protein 2,Mitogen-activated protein kinase p38 alpha (MAP kinase p38 alpha),Stress-activated protein kinase 2a (SAPK2a),CSBP,CSBP1,CSBP2,CSPB1,MXI2,SAPK2A

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0897

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-p38 MAPK (Tyr182)
分子别名

Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein (CSAID-binding protein; CSBP), MAP kinase MXI2, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha (MAP kinase p38 alpha), Stress-activated protein kinase 2a (SAPK2a), CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

Q16539
克隆号

S-617-138
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms
预测反应种属
(反应种属缩写表)

Zf, Fs, Cz, Dg, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu, Ms
IP 1:50 Hu
ICC 1:500 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu, Ms
IP	1:50	Hu
ICC	1:500	Hu

背景介绍

p38 mitogen-activated protein kinases are a class of mitogen-activated protein kinases (MAPKs) that are responsive to stress stimuli, such as cytokines, ultraviolet irradiation, heat shock, and osmotic shock, and are involved in cell differentiation, apoptosis and autophagy. Abnormal activity (higher or lower than physiological) of p38 has been implicated in pathological stresses in several tissues, that include neuronal, bone, lung, cardiac and skeletal muscle, red blood cells, and fetal tissues. Phospho-p38 MAPK (Tyr182), often tested alongside Thr180, is a significant biomarker for the activation state of p38 MAPK, a crucial enzyme in cellular stress and inflammatory responses. The phosphorylation at these sites, Thr180 and Tyr182, is essential for the full activation of p38 MAPK, allowing it to regulate various cellular processes in response to stress, inflammation, and other signals.

免疫印迹
- WB result of Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb
  Primary antibody: Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated Jurkat whole cell lysate 20 µg
  Lane 2: Jurkat treated with 25 μM Anisomycin for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 41 kDa
  Observed MW: 38 kDa
- WB result of Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb
  Primary antibody: Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 treated with 25 μM Anisomycin for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 41 kDa
  Observed MW: 38 kDa
免疫沉淀
- Phospho-p38 MAPK (Tyr182) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-p38 MAPK (Tyr182) in 0.4 mg Jurkat treated with 25 μM Anisomycin for 30 minutes whole cell lysate.
  Western blot was performed on the immunoprecipitate using Phospho-p38 MAPK (Tyr182) Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: Jurkat treated with 25 μM Anisomycin for 30 minutes whole cell lysate 40 µg (Input)
  Lane 2: Phospho-p38 MAPK (Tyr182) Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 41 kDa
  Observed MW: 38 kDa
斑点杂交
- Dot blot result of Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb
  Lane 1: Phospho-p38 MAPK (Thr180/Tyr182) peptide
  Lane 2: p38 MAPK WT peptide
  Lane 3: Phospho-p38 MAPK (Thr180) peptide
  Lane 4: Phospho-p38 MAPK (Tyr182) peptide
  Primary antibody: Phospho-p38 MAPK (Tyr182) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
- ICC analysis of Jurkat cells treated with Anisomycin (25μM, 30min) (top panel) and Jurkat cells untreated with Anisomycin (25μM, 30min) (below panel). Anti-Phospho-p38 MAPK (Tyr182) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).