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p38α MAPK+p38β MAPK Recombinant Rabbit mAb (S-1300-102)

Mitogen-activated protein kinase 14,MAP kinase 14,MAPK 14,Cytokine suppressive anti-inflammatory drug-binding protein (CSAID-binding protein,CSBP),MAP kinase MXI2,MAX-interacting protein 2,Mitogen-activated protein kinase p38 alpha (MAP kinase p38 alpha),Stress-activated protein kinase 2a (SAPK2a),CSBP,CSBP1,CSBP2,CSPB1,MXI2,SAPK2A,Mitogen-activated protein kinase 11,MAP kinase 11,MAPK 11,Mitogen-activated protein kinase p38 beta (MAP kinase p38 beta,p38b),Stress-activated protein kinase 2b (SAPK2b),p38-2,PRKM11,SAPK2,SAPK2B

浏览次数：12

货号： S0B0895

Datasheet COA

价格： ￥260
规格：
10μl25μl100μl1ml
数量：

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产品规格

宿主来源

Rabbit
抗原名称

p38α MAPK+p38β MAPK
分子别名

Mitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein (CSAID-binding protein; CSBP); MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha (MAP kinase p38 alpha); Stress-activated protein kinase 2a (SAPK2a); CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; Mitogen-activated protein kinase 11; MAP kinase 11; MAPK 11; Mitogen-activated protein kinase p38 beta (MAP kinase p38 beta; p38b); Stress-activated protein kinase 2b (SAPK2b); p38-2; PRKM11; SAPK2; SAPK2B
免疫原

Synthetic Peptide
Accession

Q16539, Q15759
克隆号

S-1300-102
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

ICFCM, IHC-P, ICC, WB, IP
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Cz, Zf, Bv
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IP	1:50
IHC-P	1:200-1:1000
ICC	1:500
ICFCM	1:500

背景介绍

p38 MAPK, a member of the mitogen-activated protein kinase (MAPK) family, is activated by a variety of environmental stresses and inflammatory cytokines. p38 MAPK family includes p38α, p38β, p38γ, and p38δ. This pathway plays a crucial role in the regulation of cellular responses to stress, inflammation, and immune function. The p38 MAPK pathway is initiated by MAPK kinase kinases (MAPKKKs), such as MEKK or MLK, which phosphorylate and activate MAPK kinases (MAPKKs) like MKK3/6. These, in turn, phosphorylate and activate p38 MAPK. Downstream of p38 MAPK, a number of substrates are regulated, including HSP27, MAPKAPK-2 (MK2), MAPKAPK-3 (MK3), and various transcription factors like ATF-2, Stat1, Max/Myc complex, MEF-2, and Elk-1. This signaling pathway is involved in a wide range of biological processes, from the regulation of gene expression to cell cycle control, apoptosis, and autophagy. The p38 MAPK pathway is also implicated in the pathogenesis of various diseases, including cancer, where it can act as either a promoter or suppressor of tumor growth and metastasis, depending on the cellular context.

免疫印迹
- WB result of p38α MAPK+p38β MAPK Recombinant Rabbit mAb
  Primary antibody: p38α MAPK+p38β MAPK Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: MCF7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 41 kDa
  Observed MW: 38 kDa
- WB result of p38α MAPK+p38β MAPK Recombinant Rabbit mAb
  Primary antibody: p38α MAPK+p38β MAPK Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse spleen lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 41 kDa
  Observed MW: 38 kDa
- WB result of p38α MAPK+p38β MAPK Recombinant Rabbit mAb
  Primary antibody: p38α MAPK+p38β MAPK Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat spleen lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 41 kDa
  Observed MW: 38 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling p38α MAPK+p38β MAPK antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling p38α MAPK+p38β MAPK antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- p38α MAPK+p38β MAPK Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating p38α MAPK+p38β MAPK in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using p38α MAPK+p38β MAPK Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: p38α MAPK+p38β MAPK Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 41 kDa
  Observed MW: 38 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-p38α MAPK+p38β antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-p38α MAPK+p38β antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-p38α MAPK+p38β MAPK antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti-p38α MAPK+p38β MAPK antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).