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宿主来源
Rabbit抗原名称
IDE分子别名
Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease (Insulinase), Insulysin免疫原
Synthetic Peptide细胞定位
Cytoplasm, Secreted, Cell membraneAccession
P14735克隆号
S-1051-61抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:4000 Hu, Ms, Rt IHC-P 1:250 Hu
Insulin-degrading enzyme (IDE) is a key enzyme involved in the breakdown of insulin and has been implicated in the pathophysiology of both type 2 diabetes and Alzheimer's disease. IDE is capable of degrading a variety of substrates, including amyloid-β peptide, which is associated with Alzheimer's disease, as well as glucagon and islet amyloid polypeptide (IAPP), all of which have significant roles in glucose metabolism. The enzyme's function extends beyond its proteolytic role, as it also exhibits chaperone activity for amyloidogenic proteins and is involved in cellular proteostasis regulation. Recent research has highlighted the potential therapeutic applications of IDE in mitigating cognitive impairment in Alzheimer's disease and diabetes. The accumulation of amyloid-β in the brain is a common feature of these conditions, and IDE's ability to degrade these plaques makes it a promising target for treatment.
免疫印迹
WB result of IDE Recombinant Rabbit mAb
Primary antibody: IDE Recombinant Rabbit mAb at 1/4000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Lane 6: SK-BR-3 whole cell lysate 20 µg
Lane 7: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 118 kDa
Observed MW: 110 kDaWB result of IDE Recombinant Rabbit mAb
Primary antibody: IDE Recombinant Rabbit mAb at 1/4000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 118 kDa
Observed MW: 110 kDaWB result of IDE Recombinant Rabbit mAb
Primary antibody: IDE Recombinant Rabbit mAb at 1/4000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Lane 3: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 118 kDa
Observed MW: 110 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-IDE antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-IDE antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-IDE antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-IDE antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-IDE antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-IDE antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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