Fetuin A Recombinant Rabbit mAb (S-1109-5)

Alpha-2-HS-glycoprotein,Alpha-2-Z-globulin,Ba-alpha-2-glycoprotein,AHSG,FETUA

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0891

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Fetuin A
分子别名

Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, AHSG, FETUA
免疫原

Recombinant Protein
细胞定位

Secreted
Accession

P02765
克隆号

S-1109-5
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IP 1:50
ICC 1:500
ICFCM 1:50

应用	稀释度
WB	1:1000
IP	1:50
ICC	1:500
ICFCM	1:50

背景介绍

Fetuin-A, also known as alpha-2-Heremans-Schmid glycoprotein (AHSG), is a multifunctional plasma glycoprotein that plays a significant role in various physiological and pathological processes. It is primarily expressed in embryonic cells and adult hepatocytes, with lower expression levels in adipocytes and monocytes. Fetuin-A is involved in the regulation of calcium metabolism, osteogenesis, and insulin signaling pathways. It acts as an inhibitor of ectopic calcification, a protease inhibitor, an inflammatory mediator, an anti-inflammatory partner, an atherogenic factor, and an adipokine, among other roles. Research has also indicated that fetuin-A plays a key role in the pathogenesis of several diseases. For instance, it has been identified as a biomarker for neurodegenerative diseases and is associated with conditions such as vascular calcification, insulin resistance, and protease activity control. Furthermore, fetuin-A has been linked to keratinocyte migration and breast tumor cell proliferative signaling.

免疫印迹
- WB result of Fetuin A Recombinant Rabbit mAb
  Primary antibody: Fetuin A Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HepG2 whole cell lysate 20 µg
  Lane 2: human serum lysate 5 µg
  Lane 3: human plasma lysate 5 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 39 kDa
  Observed MW: 50~65 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) labelling Fetuin A antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- Fetuin A Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Fetuin A in 0.4 mg human serum lysate.
  Western blot was performed on the immunoprecipitate using Fetuin A Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: human serum lysate 20 µg (Input)
  Lane 2: Fetuin A Rabbit mAb IP in human serum lysate
  Lane 3: Rabbit monoclonal IgG IP in human serum lysate
  Predicted MW: 39 kDa
  Observed MW: 50~65 kDa
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti-Fetuin A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).