CD5 Rabbit Polyclonal Antibody

T-cell surface glycoprotein CD5,Lymphocyte antigen T1/Leu-1,LEU1

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0887

规格

25μl100μl1ml

数量

产品介绍

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产品规格

背景介绍

CD5 is a cluster of differentiation expressed on the surface of T cells (various species) and in a subset of murine B cells known as B-1a. CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins. There is no confirmed ligand for CD5 but there is evidence that CD72, a C-type lectin, may be a ligand or that CD5 may be homophilic, binding CD5 on the surface of other cells. T cells express higher levels of CD5 than B cells. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. CD5 is a good immunohistochemical marker for T-cells, although not as sensitive as CD3. About 76% of T-cell neoplasms are reported to express CD5, and it is also found in chronic lymphocytic leukemia and mantle cell lymphoma (both being B cell malignancies), that do not express CD3. It is commonly lost in cutaneous T-cell lymphoma, and its absence can be used as an indicator of malignancy in this condition. The absence of CD5 in T cell acute lymphoblastic leukemia, while relatively rare, is associated with a poor prognosis.

免疫印迹
- WB result of CD5 Rabbit pAb
  Primary antibody: CD5 Rabbit pAb at 1/1000 dilution
  Lane 1: Raji whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: Molt-4 whole cell lysate 20 µg
  Negative control: Raji whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 55 kDa
  Observed MW: 50 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Raji (Human Burkitt's lymphoma B lymphocyte, left) / MOLT-4 (Human lymphoblastic leukemia T lymphoblast, right) labelling CD5 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
  Negative control: Raji
免疫沉淀
- CD5 Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating CD5 in 0.4 mg Jurkat whole cell lysate.
  Western blot was performed on the immunoprecipitate using CD5 Rabbit pAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: Jurkat whole cell lysate 20 µg (Input)
  Lane 2: CD5 Rabbit pAb IP in Jurkat whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in IP in IP in Jurkat whole cell lysate
  Predicted MW: 55 kDa
  Observed MW: 50 kDa