FUS/TLS Rabbit Polyclonal Antibody

RNA-binding protein FUS,75 kDa DNA-pairing protein,Oncogene FUS,Oncogene TLS,POMp75,Translocated in liposarcoma protein

Datasheet COA

价格￥400.00 （供应商现货： 3-5个工作日）

货号 S0B0884

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

FUS/TLS
分子别名

RNA-binding protein FUS; 75 kDa DNA-pairing protein; Oncogene FUS; Oncogene TLS; POMp75; Translocated in liposarcoma protein
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P35637
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Bv
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
ICC 1:500 Hu
ICFCM 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
ICC	1:500	Hu
ICFCM	1:50	Hu

背景介绍

FUS is a multifunctional nuclear protein that is part of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex, involved in processes such as mRNA pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. FUS belongs to the FET family of RNA-binding proteins and is implicated in various cellular processes, including gene expression regulation, maintenance of genomic integrity, and mRNA/microRNA processing. FUS plays a role in transcription regulation, RNA splicing, RNA transport, DNA repair, and damage response. It binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein, thereby coupling transcription and splicing. FUS also participates in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair. The abnormal aggregation of FUS is closely related to the occurrence and development of neurodegenerative diseases, such as Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD). Under normal conditions, FUS is located in the cell nucleus, but mutations in the gene can cause FUS to be mislocalized to the cytoplasm, where it forms inclusions, which are characteristic of certain neurodegenerative diseases.

免疫印迹
- WB result of FUS/TLS Rabbit pAb
  Primary antibody: FUS/TLS Rabbit pAb at 1/1000 dilution
  Lane 1: HepG2 whole cell lysate 20 µg
  Lane 2: K562 whole cell lysate 20 µg
  Lane 3: THP-1 whole cell lysate 20 µg
  Lane 4: SH-SY5Y whole cell lysate 20 µg
  Lane 5: Caco-2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 53 kDa
  Observed MW: 65 kDa
- WB result of FUS/TLS Rabbit pAb
  Primary antibody: FUS/TLS Rabbit pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 53 kDa
  Observed MW: 65 kDa
- WB result of FUS/TLS Rabbit pAb
  Primary antibody: FUS/TLS Rabbit pAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 53 kDa
  Observed MW: 65 kDa
流式分析
- Flow cytometric analysis of HepG2 cells labelling FUS/TLS antibody at 1/50 (1 μg) dilution/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- FUS/TLS Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating FUS/TLS in 0.4 mg K562 whole cell lysate.
  Western blot was performed on the immunoprecipitate using FUS/TLS Rabbit pAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: K562 whole cell lysate 20 µg (Input)
  Lane 2: FUS/TLS Rabbit pAb IP in K562 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in K562 whole cell lysate
  Predicted MW: 53 kDa
  Observed MW: 65 kDa
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti-FUS/TLS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).