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VISTA Recombinant Rabbit mAb (S-1469-72)

V-type immunoglobulin domain-containing suppressor of T-cell activation,Platelet receptor Gi24,Stress-induced secreted protein-1 (Sisp-1),V-set domain-containing immunoregulatory receptor,V-set immunoregulatory receptor,VSIR,C10orf54,SISP1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0882

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

VISTA
分子别名

V-type immunoglobulin domain-containing suppressor of T-cell activation; Platelet receptor Gi24; Stress-induced secreted protein-1 (Sisp-1); V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; VSIR; C10orf54; SISP1
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

Q9H7M9
克隆号

S-1469-72
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

FCM

WB

IP
稀释度

应用稀释度

WB 1:1000
IP 1:50
IHC-P 1:250
FCM 1:50

应用	稀释度
WB	1:1000
IP	1:50
IHC-P	1:250
FCM	1:50

背景介绍

VISTA is an important immune modulatory receptor that is gaining attention in cancer immunotherapy. VISTA exerts its suppressive effects on T cell activity by binding to their respective ligands on T cells, potentially influencing the immune evasion mechanisms of tumors. Studies have shown that VISTA is expressed in a variety of cancers, including melanoma, pancreatic cancer, and prostate cancer, and its expression levels are closely related to the immunosuppressive state of tumors.

免疫印迹
- WB result of VISTA Recombinant Rabbit mAb
  Primary antibody: VISTA Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: MCF7 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: DU 145 whole cell lysate 20 µg
  Lane 4: Jurkat whole cell lysate 20 µg
  Negative control: MCF7 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 34 kDa
  Observed MW: 40~55 kDa
流式分析
- Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling VISTA antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD14 - Alexa Fluor® 488 separately. Gated on total viable cells.
免疫沉淀
- VISTA Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating VISTA in 0.4 mg DU 145 whole cell lysate.
  Western blot was performed on the immunoprecipitate using VISTA Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: DU 145 whole cell lysate 20 µg (Input)
  Lane 2: VISTA Rabbit mAb IP in DU 145 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in DU 145 whole cell lysate
  Predicted MW: 34 kDa
  Observed MW: 40~55 kDa
  This blot was developed with high sensitivity substrate
免疫组化
- IHC shows positive staining in paraffin-embedded human placenta. Anti-VISTA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-VISTA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human liver. Anti-VISTA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-VISTA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-VISTA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-VISTA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-VISTA antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.