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c-IAP1 Recombinant Rabbit mAb (S-1128-137)

Baculoviral IAP repeat-containing protein 2,Cellular inhibitor of apoptosis 1 (C-IAP1),IAP homolog B,Inhibitor of apoptosis protein 2 (hIAP-2,hIAP2),RING finger protein 48,RING-type E3 ubiquitin transferase BIRC2,TNFR2-TRAF-signaling complex protein 2,BIRC2,API1,MIHB,RNF48

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0881

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

c-IAP1
分子别名

Baculoviral IAP repeat-containing protein 2, Cellular inhibitor of apoptosis 1 (C-IAP1), IAP homolog B, Inhibitor of apoptosis protein 2 (hIAP-2; hIAP2), RING finger protein 48, RING-type E3 ubiquitin transferase BIRC2, TNFR2-TRAF-signaling complex protein 2, BIRC2, API1, MIHB, RNF48
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q13490
克隆号

S-1128-137
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
ICC ? ,
WB ,
IP
稀释度

应用稀释度

WB 1:1000
IP 1:50
IHC-P 1:250
ICC 1:500

应用	稀释度
WB	1:1000
IP	1:50
IHC-P	1:250
ICC	1:500

背景介绍

c-IAP1, which stands for cellular Inhibitor of Apoptosis Protein 1, is a member of the Inhibitor of Apoptosis (IAP) family. These proteins are known for their anti-apoptotic properties and are involved in the regulation of cell survival and death pathways. c-IAP1, in particular, has been shown to inhibit apoptosis by directly binding to and inhibiting the activity of specific caspases, which are cysteine proteases that play a central role in the execution of apoptosis. Abnormal expression of IAP family members, including c-IAP1, has been associated with various cancers, suggesting their role in tumorigenesis and resistance to therapy. High expression levels of c-IAP1 in certain cancer cells have been linked to increased resistance to radiation and chemotherapy, making it a potential target for cancer therapy.

免疫印迹
- WB result of c-IAP1 Recombinant Rabbit mAb
  Primary antibody: c-IAP1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: HT-29 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 70 kDa
  Observed MW: 65 kDa
  This blot was developed with high sensitivity substrate
免疫沉淀
- c-IAP1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating c-IAP1 in 0.4 mg HepG2 whole cell lysate.
  Western blot was performed on the immunoprecipitate using c-IAP1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HepG2 whole cell lysate 20 µg (Input)
  Lane 2: c-IAP1 Rabbit mAb IP in HepG2 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
  Predicted MW: 70 kDa
  Observed MW: 65 kDa
  This blot was developed with high sensitivity substrate
免疫组化
- IHC shows positive staining in paraffin-embedded human spleen. Anti- c-IAP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti- c-IAP1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti-c-IAP1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).