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Tyrosine Hydroxylase Recombinant Rabbit mAb (S-1001-26)

Tyrosine 3-monooxygenase,Tyrosine 3-hydroxylase (TH),TH,TYH

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0880
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产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Tyrosine Hydroxylase

  • 分子别名

    Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase (TH), TH, TYH

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    P07101

  • 克隆号

    S-1001-26

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ? ,
    ICC ? ,
    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Rt
    IHC-P 1:250 Hu
    ICC 1:50 Rt
背景介绍

  • Tyrosine hydroxylase (TH) is a pivotal enzyme in the biosynthesis of catecholamines, such as dopamine, norepinephrine, and epinephrine. It catalyzes the conversion of the amino acid tyrosine to 3,4-dihydroxyphenylalanine (L-DOPA), which is the first and rate-limiting step in this biosynthetic pathway. This conversion is dependent on the cofactor (6R)-L-erythro-tetrahydrobiopterin (BH4) and ferrous iron, with the latter being directly bound to TH and oxidized to Fe3+ by O2. TH is subject to intricate regulation at both transcriptional and post-translational levels. Rapid regulation can occur through end-product inhibition influenced by the phosphorylation status of N-terminal serines, modulating the enzyme's sensitivity to the local concentration of catecholamines. Phosphorylation at specific serine residues, such as Ser40 and Ser31, can enhance TH activity and catecholamine synthesis.

  • 免疫印迹

    • WB result of Tyrosine Hydroxylase Recombinant Rabbit mAb
      Primary antibody: Tyrosine Hydroxylase Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 58 kDa
      Observed MW: 60 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human adrenal gland. Anti-Tyrosine Hydroxylase antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in PC-12 cells. Anti- Tyrosine Hydroxylase antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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